| 重组盐藻14-3-3蛋白基因的原核表达及分离纯化 |
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| 引用本文： | 王天云,昝玉玺,张俊河,张煜,王俐,郭丽.重组盐藻14-3-3蛋白基因的原核表达及分离纯化[J].四川大学学报(自然科学版),2008,45(6):1533-1536. |
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| 作者姓名： | 王天云 昝玉玺 张俊河 张煜 王俐 郭丽 |
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| 作者单位： | 新乡医学院生物化学与分子生物学教研室,新乡,453003 |
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| 基金项目： | 国家自然科学基金(30470030) |
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| 摘 要： | 根据克隆的杜氏盐藻14-3-3蛋白的基因序列设计引物，采用RT-PCR方法扩增杜氏盐藻14-3-3蛋白的基因.经原核表达、Ni2+亲和层析法纯化，免疫动物制备抗体. 抗体蛋白质印迹分析检测结果表明抗体能特异结合盐藻细胞分子量为29 kD的多肽.
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| 关 键 词： | 杜氏盐藻 14-3-3蛋白 原核表达 |
| 收稿时间： | 4/6/2007 12:00:00 AM |
Purification and prokaryotic expression of recombined Dunaliella salina 14-3-3 protein gene |
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| WANG Tian-Yun,ZAN Yu-Xi,ZHANG Jun-He,Zhang Yu,Wang Li and Guo Li.Purification and prokaryotic expression of recombined Dunaliella salina 14-3-3 protein gene[J].Journal of Sichuan University (Natural Science Edition),2008,45(6):1533-1536. |
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| Authors: | WANG Tian-Yun ZAN Yu-Xi ZHANG Jun-He Zhang Yu Wang Li and Guo Li |
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| Affiliation: | WANG Tian-Yun,ZAN Yu-Xi,ZHANG Jun-He,Zhang Yu,Wang Li,Guo Li(Department of Biochemistry , Molecular Biology,Xinxiang Medical University,Xinxiang 453003,China) |
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| Abstract: | RT-PCR was accomplished to clone 14-3-3 protein gene from Dunalilla salina.The recombinant gene was expressed in E.coli BL21 with induction of IPTG,purified by Ni2+ affinity chromatography.Fusion proteins were isolated and then lyophilized to raise the polyclonal antisera against 14-3-3 respectively by immunizing rabbits.The antibodies could bind to 29 kD polypeptides of D.salina specificity. |
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| Keywords: | Dunaliella salina 14-3-3 protein prokaryotic expression |
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