Regulation of myosin sliding alongChara actin bundles by native skeletal muscle tropomyosin |
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Authors: | T Shimmen M Yano |
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Affiliation: | (1) Faculty of Pharmaceutical Sciences, University of Tokyo, Hongo, Tokyo;(2) Department of Botany, Faculty of Science, University of Tokyo, Hongo, 113 Tokyo, Japan |
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Abstract: | Summary Native tropomyosin from rabbit skeletal muscle introduced by intracellular perfusion intoChara cells inhibited the cytoplasmic streaming irrespective of the Ca2+ concentration. To find the action site of native tropomyosin inChara, the cytoplasmic streaming was reconstituted by introducing isolated endoplasm into actin donorChara cells from which native endoplasm had been removed. The reconstituted streaming was inhibited by pretreatment of the actin donor cells with native tropomyosin but not by that of the endoplasm, suggesting that the native tropomyosin inhibited the cytoplasmic streaming by binding toChara actin bundles. Staining of the actin bundles with FITC-labeled native tropomyosin also showed that the native tropomyosin could bind to the actin bundles. Streaming reconstituted fromChara actin bundles and skeletal muscle myosin was insensitive to Ca2+, but became sensitive on application of the native tropomyosin.Abbrevations APW
artificial pond water
- ATP
adenosine 5 -triphosphoric acid
- BSA
bovine serum albumin
- EDTA
ethylene diamine tetraacetic acid
- EGTA
ethyleneglycol-bis-( -aminoethylether) N,N,N ,N -tetraacetic acid
- FITC
fluorescein isothiocyanate
- FITC-NTM
fluorescein isothiocyanate-labeled native tropomyosin
- NTM
native tropomyosin |
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Keywords: | Actin Calcium Chara Cytoplasmic streaming Myosin Native tropomyosin Tropomyosin troponin |
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