| 建立简便HBV、DNA、PCR-ELISA检测方法 |
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| 引用本文： | 詹群珊,陈禹保,林玉兵,肖文元.建立简便HBV、DNA、PCR-ELISA检测方法[J].河北医学,2002,8(7):580-582. |
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| 作者姓名： | 詹群珊 陈禹保 林玉兵 肖文元 |
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| 作者单位： | 1. 广东省深圳市人民医院,广东,深圳,518020
2. 北京美迪科生物技术有限公司,北京,100012 |
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| 摘 要： | 目的：建立一种敏感，特异的乙型肝炎病毒（HBV）DNA检测方法。方法：应用PCR扩增技术和核酸杂交技术结合酶促显色技术（即PCR-ELISA技术）来检测血清中的HBVDNA。结果：应用PCR-ELISA技术能够检出许多PCR琼脂糖凝胶电泳所检测不到的HBVDNA，大大地提高了检出率，而且，特异性强，结论：PCR-ELISA方法灵敏度高，特异强，检测结果数据化，不受主观因素的影响。
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| 关 键 词： | 乙型肝炎病毒 多聚酶链式反应 酶联免疫分析 HBVDNA检测 |
| 文章编号： | 1006-6233(2002)07-0580-03 |
Found a simple detecting method for HBV、 DNA、 PCR-ELISA |
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| ZHAN Qun-shan ,et al.Found a simple detecting method for HBV、 DNA、 PCR-ELISA[J].Hebei Medicine,2002,8(7):580-582. |
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| Authors: | ZHAN Qun-shan |
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| Affiliation: | ZHAN Qun-shan 1,et al |
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| Abstract: | Objective: A sensitive and accurate method is important for early diagnosis of patients with HBV infected. Method: We established ELISA assay for the detection of PCR amplified sequence of HBV?DNA in human serum. Result: Through this method, 268 sera from HBV positive or negative patient were detected, the results showed that the ELISA assay based on PCR product is more sensitive and more specific than electrophoresis assay. Conclusion: This method in clinical application was discussed in detailin the paper. |
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| Keywords: | HBV Polymerase chain reaction Enzyme linked immuosorbent assay |
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