| 沼泽红假单胞菌高产辅酶Q10菌株的诱变选育研究 |
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| 引用本文： | 李涵依,李建国,欧小兵,车晨,郝葆青.沼泽红假单胞菌高产辅酶Q10菌株的诱变选育研究[J].西南民族学院学报(自然科学版),2013(6):869-873. |
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| 作者姓名： | 李涵依 李建国 欧小兵 车晨 郝葆青 |
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| 作者单位： | 西南民族大学生命科学与技术学院,成都610041 |
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| 摘 要： | 以沼泽红假单胞菌为出发菌株,通过紫外诱变和罗红霉素抗性筛选,以期获得辅酶Q10高产菌株.试验结果表明,紫外照射时间60-70s时可能达到最大正向突变概率.通过紫外诱变、罗红霉素初筛和发酵复筛,获得一株沼泽红假单胞菌突变株R-1,辅酶Q10产量达到0.021g/L,较出发菌株产量（0.018g/L）提高16％.经过6次传代培养,突变菌株产辅酶Q10产量稳定,可用于进一步研究.
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| 关 键 词： | 沼泽红假单胞菌 辅酶Q10 紫外诱变 罗红霉素抗性筛选 |
Study on mutation breeding of high-yield coenzyme Q10 strain of Rhodopseudomonas palustris |
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| LI Han-yi,LI Jian-guo,OU Xiao-bing,CHE Chen,HAO Bao-qing.Study on mutation breeding of high-yield coenzyme Q10 strain of Rhodopseudomonas palustris[J].Journal of Southwest Nationalities College(Natural Science Edition),2013(6):869-873. |
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| Authors: | LI Han-yi LI Jian-guo OU Xiao-bing CHE Chen HAO Bao-qing |
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| Affiliation: | (School of Life Science and Technology, Sothwest University for Nationalities, Chengdu 610041, P.R.C.) |
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| Abstract: | Rhodopseudomonas palustris is mutagenized by means of UV radiation, and then by Roxithromycin screening, high yield eoenzyme Ql0-producing strains are selected. The result reveals that 69-70S UV radiation could obviously increase the positive mutation rate. Through UV radiation, Roxithromycin screening and re-screening with fermentation, a mutant strain R-1 is obtained. Its Q10 yield is up to 0.021g/L,which is improved by 16.6% compared with the original strain. After the sixth passage, the CoQ10 yield of the mutant strain remains steady, which shows the mutant strain could be used for further research. |
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| Keywords: | rhodopseudomonas palustris coenzyme Q10 UV mutation roxithromycin screening |
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