海棒槌胶原蛋白的酶解工艺及其产物清除自由基活性的研究

本文研究了低值的芋参科海参--海棒槌(Paracaudinachinensvar.)的胶原蛋白肽的制备、分离纯化及其清除自由基的活性。利用菠萝蛋白酶对海棒槌胶原进行酶解,通过正交试验,确定菠萝蛋白酶水解海棒槌胶原蛋白的最佳酶解条件为:酶解温度40℃,加酶量240U/ml,底物浓度18mg/100ml(以羟脯氨酸计),pH5.2,酶解时间为4h。在此试验条件下所得产物对超氧阴离子自由基的清除率可达到52.20%。采用超滤和SephadexG-25凝胶柱对酶解液进行分离纯化,得到清除超氧阴离子和羟基自由基能力较强的组分。

Study on Preparation of Free Radical Scavenging Active Peptides from Collagen Hydrolysates of Paracaudina chinens var.

The preparation,purification and characterization of free radical scavenging active peptides from collagen hydrolysates of Paracaudina chinens var. were researched. Bromelain was used to hydrolyze the sea cucumber collagen. The optimal hydrolytic conditions of sea cucumber collagen with Bromelain were determined by orthogonal test. The results show that the optimal hydrolytic conditions of Bromelain are temperature 40℃,enzyme dosage240U/ml,concentration of substrate 18mg/100ml(as hydroxyproline),pH5.2,time 4h. And the hydrolysate prepared has about 52.20% of the scavenging ratio on O·2. The high free radical scavenging active fractions were separated and purified using the ultrafiltration and Sephadex G-25.