| RP-HPLC测定八珍益母汤剂两种煎药方法芍药苷和阿魏酸的含量 |
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| 引用本文： | 冯成,王硕仁,张鹏,曹俊岭,吴爱明,娄利霞,闫彦芳,聂波. RP-HPLC测定八珍益母汤剂两种煎药方法芍药苷和阿魏酸的含量[J]. 中国现代中药, 2016, 18(5): 640-643 |
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| 作者姓名： | 冯成 王硕仁 张鹏 曹俊岭 吴爱明 娄利霞 闫彦芳 聂波 |
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| 作者单位： | 北京中医药大学 中药学院，北京100102,北京中医药大学东直门医院 中医内科学教育部和北京重点实验室，北京100700,北京中医药大学东直门医院 骨科，北京100700,北京中医药大学东直门医院 药学部，北京100700,北京中医药大学东直门医院 中医内科学教育部和北京重点实验室，北京100700,北京中医药大学东直门医院 中医内科学教育部和北京重点实验室，北京100700,北京中医药大学东直门医院 中医内科学教育部和北京重点实验室，北京100700,北京中医药大学东直门医院 中医内科学教育部和北京重点实验室，北京100700 |
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| 基金项目： | 北京市教育委员会共建项目 |
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| 摘 要： | 目的:比较传统砂锅和煎药机煎煮八珍益母汤对芍药苷和阿魏酸含量的影响。方法:采用传统砂锅和煎药机煎煮制备八珍益母汤,反相高效液相色谱(RP-HPLC)法测定煎液中芍药苷和阿魏酸的含量。色谱条件:Imtakt C18反相色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.1%磷酸(14∶86,ν/ν)溶液;流速为1.0 m L·min-1;检测波长为芍药苷230 nm,阿魏酸322 nm;柱温为25℃。结果:芍药苷在传统砂锅煎液中含量略高于煎药机煎液;阿魏酸在两种煎液中含量相近。结论:传统砂锅煎煮与煎药机煎煮八珍益母汤剂中主要有效成分含量差异不明显。
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| 关 键 词： | 传统砂锅煎煮；煎药机煎煮；八珍益母汤剂；芍药苷；阿魏酸；含量测定 |
| 收稿时间： | 2016-04-07 |
Content Determination of Paeoniflorin and Ferulic Acid in Bazhen Yimu Decoction Prepared with Two Decocting Methods by RP-HPLC |
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| FENG Cheng,WANG Shuoren,ZHANG Peng,CAO Junling,WU Aiming,LOU Lixi,YAN Yanfang and NIE Bo. Content Determination of Paeoniflorin and Ferulic Acid in Bazhen Yimu Decoction Prepared with Two Decocting Methods by RP-HPLC[J]. Modern Chinese Medicine, 2016, 18(5): 640-643 |
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| Authors: | FENG Cheng WANG Shuoren ZHANG Peng CAO Junling WU Aiming LOU Lixi YAN Yanfang NIE Bo |
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| Affiliation: | School of Chinese Materia Medica, Beijing University of Chinese Medicine,Beijing 100102,China,Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China,Department of Orthopaedics Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China,Department of Pharmacy, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China,Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China,Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China,Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China and Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China |
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