Effects of exogenous nitric oxide on antioxidation and DNA methylation of <Emphasis Type="Italic">Dendrobium huoshanense</Emphasis> grown under drought stress |
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Authors: | Honghong Fan Tingchun Li Lei Guan Zhengpeng Li Ning Guo Yongping Cai Yi Lin |
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Affiliation: | (1) Department of Life Sciences, Anhui Agricultural University, Hefei, 230036, People’s Republic of China;(2) Anhui Academy of Agricultural Sciences, Hefei, 230031, People’s Republic of China |
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Abstract: | Nitric oxide (NO) is an important biological messenger in plants, which has been implicated in response to abiotic stress.
To study the effects of exogenous NO on drought menace, the tube seedlings of Dendrobium huoshanense were selected and treated with 10% polyethylene glycol (PEG-6000) to simulate drought stress. After application of sodium
nitroprusside (SNP), the relative water content (RWC) and antioxidant enzyme activities were determined. As a result, plant
treated with 50 μmol L−1 of SNP maintained high level of RWC and lower content of malondialdehyde (MDA). Furthermore, the antioxidant enzyme activities
were obviously enhanced. However, the higher concentration of SNP (100 μmol L−1) enhanced the effects of drought stress for plant. For further analysis of the response mechanism to exogenous NO, the methylation-sensitive
amplified polymorphism (MSAP) technique was used to investigate the changes of DNA methylation. When the seedlings of Dendrobium huoshanense were treated with 50 μmol L−1 SNP containing 10% PEG-6000, levels of global DNA methylation of Dendrobium huoshanense were decreased. Nevertheless, the demethylation rate of methylated sites increased, accounting for 12.5% of total methylation
sites. These results implied that some expressed genes were involved in the response process to drought stress triggered by
NO in Dendrobium huoshanense. |
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