嗜酸乳杆菌NCFM胞外多糖对小鼠免疫相关基因的调控

目的：研究嗜酸乳杆菌(Lactobacillus acidophilus)NCFM胞外多糖对小鼠免疫相关基因的调控作用。方法：通过构建小鼠模型，对小鼠脏器系数进行分析，提取肠组织RNA，采用Real-time RT-PCR方法对免疫相关基因chemokine (C-C motif) ligand 2(CCL2)和interleukin 18(IL18)的表达情况进行分析。结果：脏器系数随着天数的增加而增加，实验组的脏器系数较对照组相比，显著增加。在2个免疫相关基因中，随着时间的延长，IL18和CCL2呈现出先迅速增加后减少的趋势，同时均在第4天表达量达到最大值，且与对照组相比差异极显著(P＜0.01)。结论：胞外多糖可以提高小鼠免疫脏器的质量，并且能够引起免疫相关基因出现特定表达变化，对小鼠机体的免疫调控具有一定的作用。

Regulation of Exopolysaccharide Produced by Lactobacillus acidophilus NCFM on Immune-Related Genes in Mice

Objective: Exopolysaccharide (EPS) is a metabolic product of probiotics with antitumor, immunomodulatory andantioxidant functions. The aim of this study is to explore the effect of EPS produced by Lactobacillus acidophilus NCFMon immune-related genes in mice. Methods: Viscera coefficients were analyzed through mouse model. Real-time PCR wasused to evaluate the expression of immune-related genes in mice after extracting total RNA from intestinal tissues. Results:The results indicated that the viscera coefficients revealed an increase with the extension of time and a significant differencein viscera coefficients between experimental group and control group (P＜0.01). The expression of immune-related genessuch as CCL2 and IL18 genes exhibited an initial increase and a final decrease with the extension of time and had the highestlevel on the 4th day. Similarly, a significant difference in the expression of these genes between experimental group andcontrol group was observed (P＜0.001). Conclusion: EPS can increase the relative weight of immune viscera, and affect thedifferential expression of immune-related genes. EPS produced by L. acidophilus NCFM can enhance immune function.