不同无性系杨树叶片的超氧物歧化酶_SOD_同工酶谱比较研究

本文对不同无性系杨树叶片的超氧物歧化酶（ＳＯＤ）同工酶谱进行了比较研究，实验结果表明：不同无性系杨树叶片ＳＯＤ同工酶谱都具有较小迁移率的Ｓ带、中等迁移率的Ｍ带和较大迁移率的Ｆ带；Ｓ带为Ｍｎ－ＳＯＤ，Ｍ带和Ｆ带为Ｃｕ、Ｚｎ－ＳＯＤ．各无性系ＳＯＤ同工酶谱都具有１条Ｓ带和１条Ｍ带，Ｆ带数目在各无性系存在差异；青杨和黑杨的Ｆ带为３条，白杨的Ｆ带为６条，亲缘关系较近的青杨和黑杨ＳＯＤ同工酶谱较为相似，而与它们亲缘关系较远的白杨ＳＯＤ同工酶谱存在较大差异，ＳＯＤ同工酶谱分析可应用于杨属植物的分组、无性系及亲缘关系的鉴定。

COMPARATIVE STUDY ON SUPEROXIDE DISMUTASE ISOENZYMES ZYMOGRAM IN THE LEAVES OF DIFFERENT POPLAR CLONES

This paper is a comparative study on superoxide dismutase(SOD)isoenzymes zymogram in leavesof different Poplar clones.Results are as follows: all poplar clones measured in this experimenthad 1 band of slowest mobility(S band), 1 band of medium mobility(M band)and 3 or 6 bandsof fastest mobility(F band).5 band was proved to be Mn-SOD, while the others(M and F bands)were Cu , Zn-SOD. SOD isoenzymes zymogram of Poplar clone was the same at differ-ent growth stages. In spite of slight changes in activity of poplar,there were a great similarity inthe same section of poplar. Different sections of Poplar clones had different numbers of F bandsin SOD isoenzymes zymogram. Aigeiros and Tacamachaca,which have a lose inheritable relation-ship, contain 3 F bands, while Populus has 6 F bands. SOD isoenzymes zymogram in differentPoplar clones,having inheritable particularity of species,can be used to classify Poplar clones andidentify their inheritable relationships.