高通量测序分析妊娠期糖尿病对早产儿肠道菌群的影响

目的 通过高通量测序技术探究妊娠期糖尿病对早产儿肠道菌群的影响,并对相关功能及代谢通路进行预测。 方法 选取符合入组条件的早产儿40例,依据孕母是否患有妊娠期糖尿病分为妊娠期糖尿病组(GDM组)和对照组,各20例,于出生后24 h内收集早产儿胎便进行16S rRNA基因测序,对测序结果进行物种分类学分析及生物信息学分析。 结果 GDM组显示出更低的物种丰富度及多样性(P=0.048)。两组物种组成存在显著差异(P=0.048),GDM组厚壁菌门与拟杆菌门比值明显升高,机会致病菌相对丰度增加,同时革兰阴性杆菌比例上升。PICRUSt功能预测分析表明,GDM组在碳水化合物转运及代谢、细胞外结构、环境信息处理代谢通路和免疫性疾病代谢通路的功能丰度增高。 结论 妊娠期糖尿病可导致早产儿肠道微生态平衡被破坏,肠道菌群失调或为母亲患有GDM的早产儿多种疾病发病率增高的原因之一。

High throughput sequencing analysis of the effects of gestational diabetes mellitus on intestinal flora in premature infants

Objective To explore the effect of gestational diabetes mellitus(GDM) on intestinal flora of premature infants using high throughput sequencing analysis, and speculate the related functions and metabolic pathways. Methods Forty premature infants who met the criterion were divided into GDM group or control group with 20 cases in each group according to whether the mother had GDM or not. Meconium samples were collected within 24 hours after birth for 16S rRNA gene sequencing, and the sequencing results were analyzed with taxonomic analysis and biological information analysis. Results The GDM group showed lower species richness and diversity than the control group(P=0.048). There was significant difference in species composition between the two groups(P=0.048). The ratio of Firmicutes to Bacteroidetes in GDMgroup increased significantly, and the proportion of opportunistic pathogens and Gram negative bacilli increased. PICRUSt functional prediction analysis showed that the abundances in carbohydrate transport and metabolism, extracellular structure, environmental information processing and immune disease increased in the GDM group. Conclusion GDM can lead to the destruction of intestinal microecology balance in premature infants. Intestinal flora imbalance may be one of the reasons for the increased incidence of various diseases in infants of mothers with GDM.