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| HPLC法测定阿昔洛韦片中阿昔洛韦的含量 | |
| 引用本文： | 范玉峰,李恒,胡守莲. HPLC法测定阿昔洛韦片中阿昔洛韦的含量[J]. 中国医药导报, 2008, 5(28): 23-24 |
| 作者姓名： | 范玉峰 李恒 胡守莲 |
| 作者单位： | 1. 信阳职业技术学院附属医院,河南信阳,464000 2. 信阳职业技术学院药学系,河南信阳,464000 3. 河南省信阳市食品药品检验所,河南信阳,464000 |
| 摘 要： | 目的：建立阿昔洛韦片中阿昔洛韦的含量测定方法。方法：采用高效液相色谱法，以十八烷基硅烷键合硅胶为填充剂，色谱柱为HypersilC18柱（4．6mm×200mm，5μm）；流动相为甲醇-水（10：90）；流速1．0ml／min；检测波长254nm；柱温：室温；进样量20μl。按外标法以峰面积计算阿昔洛韦含量（以标示量的百分含量表示）。结果：阿昔洛韦浓度在5-30μg／ml范围内与峰面积呈良好的线性关系；平均回收率为100．00％，RSD=0.22％；三批样品的平均含量是98．9％，RSD=0．20％。结论：本方法专属性强、灵敏度高、重现性好，可用于阿昔洛韦的含量测定。 |
| 关 键 词： | 阿昔洛韦片 含量测定 HPLC |
Content determination of acyclovir in Acyclovir Tablets by HPLC |
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| FAN Yu-feng,LI Heng,HU Shou-lian. Content determination of acyclovir in Acyclovir Tablets by HPLC[J]. China Medical Herald, 2008, 5(28): 23-24 | |
| Authors: | FAN Yu-feng LI Heng HU Shou-lian |
| Affiliation: | FAN Yu-feng, LI Hen, HU Shou-lian (1.Affiliated Hospital of Xinyang professional technology college,Xinyang 464000,China;2. Pharmaceutical Department, Xinyang professional technology college, Xinyang 464000,China;3.Xinyang Institute for Food and Drag Control, Xinyang 464000,China) |
| Abstract: | Objective：To establish a method for the content determination of acyclovir. Methods：HPLC method was adopted with Hypersil Cls column（4.6 mm×200 mm,5μm）, the mobile phase was methanol-water （10：90） and flow rate was 1.0 ml/min.The detector wavelength was 254 nm. External standard method was used for the content calcaulation. Results： Acyclovir had a good linearity between 5~30 μg/ml. The average recovery was 100.00% with RSD of 0.22%. The average content of the three samples was 98.9% with RSD of 0.20%. Conclusion：The method has good specifity,sensitivity and reproducibility, can be used for the content determination of acyclovir. |
| Keywords: | HPLC |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
