Expanding an Efficient,Electrically Driven and CNT‐Tagged P450 System into the Third Dimension: A Nanowired CNT‐Containing and Enzyme‐Stabilising 3 D Sol–Gel Electrode |
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Authors: | Dr. Enrique C. Llaudet Dominique Darimont Dr. Ramona Samba Ilona Matiychyn Dr. Martin Stelzle Dr. Martin J. Weissenborn Prof. Dr. Bernhard Hauer |
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Affiliation: | 1. NMI Natural and Medical Sciences Institute, University of Tübingen, Reutlingen, Germany;2. Institute of Technical Biochemistry, University of Stuttgart, Stuttgart, Germany |
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Abstract: | Although electrochemically catalysed P450 reactions have been described, their efficiency and applicability remained limited. This is mostly due to low enzyme activity, laborious protein immobilisation and the small electrode surface. We established a novel protein immobilisation method for a determined orientation and electrical wiring of the enzyme without post‐expression modification. By genetic introduction of an anchor‐peptide our method is applicable for screening medium to large mutant libraries and detection by an electrode system. The system was expanded by using wired carbon nanotubes within a sol‐gel matrix to create a three dimensional electrode. |
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Keywords: | assay bio-electrochemistry electrode electrochemistry enzyme catalysis nanotubes P450 |
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