Erythrocyte membranes - compression of lipid phases by increased cholesterol content |
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Authors: | John Kroes Rosemarie Ostwald Alec Keith |
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Affiliation: | 1. Department of Nutritional Sciences, University of California, Berkeley, Calif. 94720 U.S.A.;2. Department of Genetics, University of California, Berkeley, Calif. 94720 U.S.A. |
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Abstract: | Lipid and protein interactions were studied in guinea pig erythrocytes containing a normal or a two-fold increased amount of cholesterol. The electron spin resonance (ESR) spectra of cholesterol-loaded cells labeled with fatty-acid probes showed an increase in the local viscosity of the membrane as compared with control cells. This increase reflects changes in the interior of the lipid matrix of the membrane because the probes resisted destruction by ascorbate, were unaffected by the action of pronase, and gave spectra similar to those of liposomes. No differences were observed between control and cholesterol-loaded cells in the conformation of the membrane proteins by either the infrared spectra or the ESR spectra of cells labeled with maleimide probes. |
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Keywords: | ESR electron spin resonance |
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