| 高效液相色谱法测定复方拳参片中橙皮苷的含量 |
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| 引用本文： | 王海波,宋汉敏,李振国. 高效液相色谱法测定复方拳参片中橙皮苷的含量[J]. 中国实验方剂学杂志, 2012, 18(14): 122-123 |
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| 作者姓名： | 王海波 宋汉敏 李振国 |
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| 作者单位： | 河南省食品药品检验所,郑州,450003 |
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| 摘 要： | 目的:建立复方拳参片中橙皮苷的高效液相色谱含量测定方法.方法:C18色谱柱Phenomenex Luna (4.6 mm×250 mm,5μm),柱温30℃,流动相甲醇-醋酸-水(35∶4∶61),流速1.0 mL· min -1,检测波长283 nm.结果:橙皮苷的线性范围为104·1040ng,平均加样回收率为97.1％(n=6).结论:方法简便、准确,可用于复方拳参片中橙皮苷的含量测定和产品质量控制.
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| 关 键 词： | 复方拳参片 橙皮苷 高效液相色谱法 |
| 收稿时间： | 2012-03-12 |
Determination of Hesperidin in Fufang Quanshen Tablet by RP-HPLC |
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| WANG Hai-bo,SONG Han-min and LI Zhen-guo. Determination of Hesperidin in Fufang Quanshen Tablet by RP-HPLC[J]. China Journal of Experimental Traditional Medical Formulae, 2012, 18(14): 122-123 |
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| Authors: | WANG Hai-bo SONG Han-min LI Zhen-guo |
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| Affiliation: | Henan Institute for Food and Drug Control,Zhengzhou 450003,China;Henan Institute for Food and Drug Control,Zhengzhou 450003,China;Henan Institute for Food and Drug Control,Zhengzhou 450003,China |
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| Abstract: | Objective:To develop an RP-HPLC method for determination of hesperidin in Fufang Quanshen tablet.Method: A Phenomenex Luna C18 column(4.6 mm×250 mm,5 μm)was applied;a mobile phase composing of methanol-acetic acid-water(35∶4∶61) was used,and the flow rate was 1.0 mL·min-1;the UV detector was set at 283 nm.Result:The linear range of hesperidin was 104-1 040 ng,and the average recovery was 97.1%(n=6).Conclusion:A sensitive,simple RP-HPLC method was established for the determination of hesperidin in Fufang Quanshen tablet. |
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| Keywords: | Fufang Quanshen tablet hesperidin RP-HPLC |
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