柿叶不同极性部位对H22腹水瘤及H22,S180实体瘤小鼠的抑瘤作用

目的:研究柿叶不同极性部位对小鼠H22及S180抑瘤作用的影响。方法:柿叶乙醇提取物分别用三氯甲烷、乙酸乙酯、正丁醇萃取,得到相应不同极性部位;乙醇提取后的柿叶晾干后水煮,得水提部位。分别建立H22腹水瘤小鼠模型和H22,S180实体瘤小鼠模型,将荷瘤小鼠随机分为空白组,模型组,环磷酰胺组(0.1 g·kg-1),不同极性部位(柿叶三氯甲烷、柿叶水提、柿叶乙酸乙酯、柿叶正丁醇)高、中、低剂量组,连续ig 10 d。观察各用药组对H22腹水瘤小鼠腹围、腹水抑制率和肝组织病理学形态的影响,以及对H22和S180实体瘤小鼠摄食量、体重、抑瘤率、生命延长率、胸腺和脾脏系数等的影响。结果:与模型组比较,柿叶乙酸乙酯部位、水提部位和正丁醇部位能明显减少H22腹水瘤小鼠的腹水量,不同程度改善肝细胞肿胀、溶解等状况;不影响H22和S180实体瘤小鼠的摄食量,能明显提高荷瘤小鼠的抑瘤率、延长生命延长率,增加胸腺系数和脾脏系数(P<0.01,P<0.05)。结论:柿叶乙酸乙酯部位、水提部位和正丁醇部位对H22和S180荷瘤小鼠有不同程度的抑瘤作用,可为柿叶抑瘤作用的有效部位。柿叶乙酸乙酯部位对肝癌H22小鼠(包括实体瘤和腹水瘤)的影响比对肉瘤S180小鼠更显著。

Antitumor Effects of Different Polarity Extracts from The Kaki Folium on Hepatoma H22 and Sarcoma H22,S180 Bearing Mice

Objective: To observe the antitumor effects of different polarity extracts from Kaki Folium on hepatoma H22 and sarcoma S180 bearing mice. Method: The the Kaki Folium were successively extracted with ethanol, chloroform, ethyl acetate and n-butanol, the ethanol extraction were extracted by water, and the different polarity extracts were obtained. Experimental H22 ascites tumor mice model, H22 and S180 solid tumor mice models were established, respectively. The experimental mice were randomly divided into a series of groups:blank control group, model group, cyclophosphamide group, low-, middle-and high-dosage groups of the various different polarity extracts. The mice were intragastrically administrated the corresponding drugs for 10 days. The abdominal circumference, inhibition rate of ascites and liver tissue physiological form were observed in H22 ascites tumor mice. The food intake, body weight, tumor inhibition rate, life prolongation rate, values of thymus coefficient and spleen coefficient were recorded. Result: Compared with the model group, the volume of ascites and ameliorate liver cell swelling, karyolysis on H22 ascites tumor mice were significantly reduced in ethyl acetate extract, water extract and n-butanol extract groups, while no significant effects on food intake and body weight in H22, S180solid tumor mice were found. The tumor inhibition rate, life prolongation rate, values of thymus coefficient and spleen coefficient were significantly increased (P<0.01 or P<0.05) after treated with different polarity extracts and cyclophosphamide groups. Conclusion: The ethyl acetate extract, water extract and n-butanol from Kaki Folium possess have certain antitumor effects on hepatoma H22 and sarcoma S180 bearing mice, which may be the effective fractions of antitumor effects from persimmon leaves. The ethyl acetate-extracted fraction has better effects on hepatoma H22 bearing mice (included the solid and ascites tumor) than sarcoma S180 bearing mice.