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| 荧光检测孕妇感染蛋白质芯片制备的初步研究 | |
| 引用本文： | 张茜,张春秀,蒋犁,唐祖民,陆祖宏,蒋涛.荧光检测孕妇感染蛋白质芯片制备的初步研究[J].东南大学学报(医学版),2006,25(1):9-11. |
| 作者姓名： | 张茜 张春秀 蒋犁 唐祖民 陆祖宏 蒋涛 |
| 作者单位： | 1. 东南大学附属中大医院,儿科,江苏,南京,210009 2. 东南大学,生物科学与医学工程系,江苏,南京,210096 3. 南京市妇幼保健院,江苏,南京,210004 |
| 摘 要： | 目的：建立一种用蛋白质芯片检测血清中弓形虫、巨细胞病毒、风疹病毒、单纯疱疹病毒（TORCH）抗体的新方法。方法：采用玻片表面修饰琼脂糖膜制备蛋白质芯片，以荧光标记抗体，检测血清中TORCH IgM抗体。结果：所制备的蛋白质芯片可检测到血清中微量TORCH抗体的存在，荧光强度和抗原质量浓度呈线性关系，抗体之间交叉反应可忽略不计。该芯片法与ELISA试荆盒检测阴阳性符合率均100％。结论：蛋白质芯片技术检测孕妇TORCH感染，具有高通量、微型化、操作简便等优点，可望应用于临床中。 |
| 关 键 词： | 风疹病毒 弓形虫属 巨细胞病毒 疱疹病毒2型 人 蛋白质阵列分析 妊娠并发症感染性 妊娠 |
| 文章编号： | 1671-6264(2006)01-0009-03 |
| 修稿时间： | 2004年12月6日 |
Primary study in preparation of protein microarray in pregnancy infection |
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| ZHANG Qian,ZHANG Chun-xiu,JHANG Li,TANG Zu-min,LU Zu-hong,JIANG Tao.Primary study in preparation of protein microarray in pregnancy infection[J].Journal of Southeast Univ: Medical Sci Ed,2006,25(1):9-11. | |
| Authors: | ZHANG Qian ZHANG Chun-xiu JHANG Li TANG Zu-min LU Zu-hong JIANG Tao |
| Abstract: | Objective To develop a new method of TORCH protein microarray for detecting TORCH infections.Methods The antigen were printed on glass slide modified with the agarose films.The serum antibodies against the TORCH antigen were detected by using fluorescence labeled secondary antibodies.Results TORCH protein microarray could detect minimum antibodies against the TORCH antigen.The cross-reactivities of the anti-IgM goat were negligible.100% concordance were observed between microarray and ELISAs.Conclusion The TORCH protein microarray,having the advantages of high throughput,convenience,micromation,will be used in clinic in the future. |
| Keywords: | rubella virus toxoplasma cytomegalovirus herpesvirus 2 human protein array analysis pregnancy complications infectious pregnancy |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
