Thermostable ethanol tolerant xylanase from a cold-adapted marine species Acinetobacter johnsonii |
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Authors: | Dongsheng Xue Xuhao Zeng Dongqiang Lin Shanjing Yao |
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Affiliation: | 1.Key Laboratory of Fermentation Engineering(Hubei Universty of Technology), Ministry of Education, Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei Key Laboratory of Industrial Microbiology, Hubei University of Technology, Wuhan 430068, China;2.Department of Chemical and Bioengineering, Zhejiang University, Hangzhou 310027, China |
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Abstract: | A xylanase-producing bacterium, isolated from deep sea sediments, was identified as the cold-adapted marine species Acinetobacter Johnsonii. A cold-adapted marine species Acinetobacter Johnsonii could grow at 4℃. The optimum temperature and pH of xylanase from a cold-adapted marine species Acinetobacter Johnsonii were 55℃ and pH 6.0. Xylanase from a cold-adapted marine species Acinetobacter Johnsonii remained at 80% activity after incubation for 1 h at 65℃. The xylanase activity was 1.2-fold higher in 4% ethanol solution than in ethanol free solution. Gibbs free energy of denaturation, ΔG, was higher in 4% ethanol solution than in ethanol free solution. Thermostable ethanol tolerant xylanase was valuable for bioethanol production by simultaneous saccharification and fermentation process with xylan as a carbon source. |
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Keywords: | Xylanase Ethanol tolerant Thermostable Cold-adapted Acinetobacter Johnsonii |
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