高效液相色谱整体柱在药物分离分析中的应用进展

本文对高效液相整体柱在药物分离分析方面的应用进行了综述.主要介绍了以烷氧基硅烷为主要原料,采用溶胶-凝胶法制备的硅胶整体柱,由于其具有微米级通孔结构和大的比表面积,他们在高效、快速分离小分子物质方面得到广泛地应用.对于聚合物整体柱,主要介绍了包括分子印迹聚合物在内的有机聚合物整体柱在药物分离、生物样品的处理等方面的应用.

Application and development of monolithic stationary phases for HPLC in pharmaceutical analysis and separation

In recent years,monolithic supports as stationary phases in high-performance liquid chromatography（HPLC） have become a rapidly burgeoning field.This review summarizes the current achievements and their application in pharmaceutical analysis and separation.One kind is the silica monoliths prepared from tetraalkoxysilane by a sol-gel method with either micrometer-size through-pores or high specific surface areas and well suited for small molecules in HPLC modes.The other kind is the organic polymer-based monoliths including monolithic molecularly imprinted polymers in treatments of the samples before injected into the chromatograph in biological fluids.The analytical potential of these columns is demonstrated with separations involving various drugs in different chromatographic modes.