缺血预处理对大鼠冷缺血再灌注损伤的保护作用的实验研究

目的：探讨缺血预处理对大鼠肾脏冷缺血再灌注损伤的保护作用。方法：36只SD大鼠制备成肾脏冷缺血再灌注损伤模型，随机分为假手术组（A组）、冷缺血再灌注组（B组）、缺血预处理组（C组），免疫组化观察Bcl2、Bax蛋白表达，测定肾功能、肾组织超氧化物歧化酶、丙二醛，病理切片观察肾组织损伤形态学的变化。结果：B组、C组BUN、CR测定值均显著高于A组，而C组低于B组，差异具有显著性（P〈0.01）；C组大鼠肾组织超氧化物酯化酸水平较B组显著升高，而丙二醛水平显著性下降（P〈0．01）；B组、C组Bcl2、Bax蛋白较A组均明显增加，C组较B组Bcl—2表达显著增强，Bax表达明显下降（P均〈0．01），肾组织损伤的病理分级显著减轻。结论：缺血预处理对肾脏冷缺血再灌注性损伤具有保护作用，其机制可能与上调Bcl2和下调Bax蛋白表达相关。

Experimental study on protective effect of ischemic preconditioning on renal cold ischemia reperfusion injury in rat

Objective To investigate the protective effect of ischemic preconditioning on rat with renal cold ischemia reperfusion injury.WTHZ]Methods Thirty-six SD rats with cold ischemia reperfusion injury were randomly divided into the following 3 groups: the sham operated group(A),cold ischemia reperfusion group(B),and treatment group(C) receiving ischemic(5 minutes) and reperfusion(5 minutes) three times.The expression of Bcl-2 and Bax protein were observed with immunohistochemistry.The level of renal function,SOD,MDA and histologic examination were detected.Results Blood urea nitrogen and creatinine levels in group B and C were significantly higher than in group A(P<0.01),group C was lower than group B(P<0.01).SOD in group C was significantly higher than in group B(P< 0.01),but MDA decreased significantly(P<(0.01)).Bcl-2 and Bax were all increased in group B and group C as compared with that of group A(P<0.01).Bcl-2 was increased but Bax was decreased significantly in group C than in group B(P<0.01).Group C showed a less pathological change and a lighter renal impairment(P<0.01).Conclusion Ischemic preconditioning has protective effect on renal cold ischemia reperfusion injury through regulating Bcl-2 and Bax expression.