Electrical protein array chips for the detection of staphylococcal virulence factors |
| |
Authors: | Annett Quiel Britta Jürgen Gundula Piechotta Anne-Pascale Le Foll Anne-Kathrin Ziebandt Christian Kohler Daniela Köster Susanne Engelmann Christian Erck Rainer Hintsche Jürgen Wehland Michael Hecker Thomas Schweder |
| |
Affiliation: | 1. Pharmazeutische Biotechnologie, Institut für Pharmazie, Ernst-Moritz-Arndt-Universit?t, Friedrich-Ludwig-Jahn-Str. 17, 17487, Greifswald, Germany 2. Fraunhofer Institut für Siliziumtechnologie, Fraunhoferstr. 1, 25524, Itzehoe, Germany 3. Institut Scientifique d’Hygiène et d’Analyse (ISHA), Rue du Chemin Blanc-Champlan, 91165, Longjumeau, France 4. Institut für Mikrobiologie und Molekularbiologie, Ernst-Moritz-Arndt-Universit?t, Friedrich-Ludwig-Jahn-Str. 15, 17487, Greifswald, Germany 5. Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124, Braunschweig, Germany 6. AJ eBiochip GmbH, Fraunhoferstr. 1, 25524, Itzehoe, Germany
|
| |
Abstract: | A new approach for the detection of virulence factors of Staphylococcus aureus and Staphylococcus epidermidis using an electrical protein array chip technology is presented. The procedure is based on an enzyme-linked sandwich immunoassay, which includes recognition and binding of virulence factors by specific capture and detection antibodies. Detection of antibody-bound virulence factors is achieved by measuring the electrical current generated by redox recycling of an enzymatically released substance. The current (measured in nanoampere) corresponds to the amount of the target molecule in the analyzed sample. The electrical protein chip allows for a fast detection of Staphylococcus enterotoxin B (SEB) of S. aureus and immunodominant antigen A homologue (IsaA homologue) of S. epidermidis in different liquid matrices. The S. aureus SEB virulence factor could be detected in minimal medium, milk, and urine in a concentration of 1 ng/ml within less than 23 min. Furthermore, a simultaneous detection of SEB of S. aureus and IsaA homologue of S. epidermidis in a single assay could be demonstrated. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|