| Src激酶抑制剂FB2抗前列腺癌作用研究 |
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| 引用本文： | 张燕,李洪燕,张翼,苏富琴,冯志强,陈晓光.Src激酶抑制剂FB2抗前列腺癌作用研究[J].哈尔滨商业大学学报(自然科学版),2009,25(2). |
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| 作者姓名： | 张燕 李洪燕 张翼 苏富琴 冯志强 陈晓光 |
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| 作者单位： | 中国医学科学院,北京协和医学院,药物研究所,北京,100050 |
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| 摘 要： | 探讨Src激酶抑制剂FB2抗前列腺癌的作用及机制.整体动物实验观察FB2对人前列腺癌PC-3细胞在裸鼠异体中生长的影响;MMT、克隆原形成、细胞黏附试验观察FB2对肿瘤细胞增殖、黏附的影响;流式细胞术分析FB2对细胞周期的影响;Western blot观察细胞及组织内Src及p-Src表达.实验结果表明FB2抑制PC-3细胞裸鼠移植瘤的生长;FB2抑制PC-3细胞增殖及对基底膜成分的黏附,并将细胞周期阻滞于G1期;机制研究表明,FB2抑制PC-3细胞及瘤组织中Src激酶Tyr416磷酸化.因此Src激酶抑制剂FB2在体内外均有较强的抗前列腺癌作用,其机制可能与抑制Src激酶Tyr416磷酸化有关.
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| 关 键 词： | 前列腺癌 Src激酶 Src激酶抑制剂 |
Anticancer activity of FB2,a Src kinase inhibitor,on prostate cancer |
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| ZHANG Yan,LI Hong-yan,ZHANG Yi,SU Fu-qin,FENG Zhi-qiang,CHEN Xiao-guang.Anticancer activity of FB2,a Src kinase inhibitor,on prostate cancer[J].Journal of Harbin University of Commerce :Natural Sciences Edition,2009,25(2). |
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| Authors: | ZHANG Yan LI Hong-yan ZHANG Yi SU Fu-qin FENG Zhi-qiang CHEN Xiao-guang |
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| Affiliation: | Institute of Materia Medica;Chinese Academy of Medical Sciences & Peking Union Medical College;Beijing 100050;China |
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| Abstract: | Aim at evaluate the anticancer activity of FB2,a Src kinase inhibitor,on prostate cancer.Animal experiments are used to determine the antitumor activity of FB2 on PC-3 xenograft tumor growth in vivo.MTT assays,colony formation and adhesion assays are utilized to assess impact of FB2 on the cell proliferation and cell adhesion.Flow cytometry is used to analysis cell cycle distribution.Western blot is used to analysis the level of phosphorylation of Src at Tyr 416 and Src.FB2 had significant antitumor activit... |
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| Keywords: | prostate cancer src kinase src kinase inhibitor |
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