| p38MAPK在低能ESW和间歇rhPTH1-34促进ROB成骨的信号转导中的作用 |
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| 引用本文： | 王 李,刘长剑,罗宗键. p38MAPK在低能ESW和间歇rhPTH1-34促进ROB成骨的信号转导中的作用[J]. 大连医科大学学报, 2011, 33(1): 31-35 |
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| 作者姓名： | 王 李 刘长剑 罗宗键 |
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| 作者单位： | 王李,WANG Li(大连市友谊医院医学影像科,辽宁,大连,116001);刘长剑,LIU Chang-jian(大连医科大学附属第一医院,骨科,辽宁大连,116011);罗宗键,LUO Zong-jian(长春中医药大学附属医院,骨科,吉林,长春,130021) |
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| 摘 要： | [目的]探讨低能体外冲击波(ESW)和间歇人重组甲状旁腺素(rhPTH1-34)刺激引起的体外培养大鼠成骨细胞(ROB)成骨的细胞内信号转导中p38MAPK的作用.[方法]分别用有效的低能ESW刺激和间歇rhPTH1-34作用于ROB,并设立加入p38MAPK抑制剂SB203580组,来观察ROB增殖、成骨指标及Wes...
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| 关 键 词： | 成骨细胞 体外冲击波 rhPTH1-34 p38MAPK |
Role of p38MAPK in signal transduction of low density ESW and low dose intermittent rhPTH1-34's action on bone formation of cultured rat osteoblasts |
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| WANG Li,LIU Chang-jian and LUO Zong-jian. Role of p38MAPK in signal transduction of low density ESW and low dose intermittent rhPTH1-34's action on bone formation of cultured rat osteoblasts[J]. Journal of Dalian Medical University, 2011, 33(1): 31-35 |
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| Authors: | WANG Li LIU Chang-jian LUO Zong-jian |
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| Affiliation: | 1.Department of Medical Radiology,the Friendship Hospital of Dalian,Dalian 116001 China;2.Orthopedic department,the First Affiliated Hospital of Dalian Medical University,Dalian 116011 China;3.Orthopedic department,Affiliated Hospital of Changchun Chinese Medicine University,Changchun 130021 China) |
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| Abstract: | [Objective] To investigate the role of p38MAPK in the signal path way of low density ESW and low dose intermittent rhPTH1-34 stimulation′s action on rat osteoblasts.[Methods] After stimulations of 0.18 mJ/mm2 ESW and 10-11mol/L intermittent rhPTH1-34,the specific inhibitor of p38MAPK was added for finding the role of p38MAPK in the intracellular signal path way.The rat osteoblasts were collected,and cultured was detected,proliferation of these cells was observed by MTT and bone formation by measuring ALP activity.The expression of p-p38MAPK was detected by Western blot.[Results] Enhaning effect on bone formation by ESW can be significantly inhibited by SB203580,a inhibitor of p38MAPK(P<0.05).But the enhancement of ROBs’ bone formation by intermittent rhPTH1-34 can not be inhibited by SB203580.Suitable ESW stimulation can improve the expression of p38MAPK,but intermittent rhPTH1-34 stimulation can not.[Conclusions] Suitable ESW stimulation can enhance bone formation of ROB by activation of p38MAPK.The enhancement of ROBs’ bone formation by intermittent rhPTH1-34 stimulation dosen’t depend on activation of p38MAPK. |
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| Keywords: | rhPTH1-34 p38MAPK |
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