| 核酸酶P_1固定化研究 |
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| 引用本文： | 刘红 潘红春. 核酸酶P_1固定化研究[J]. 食品与发酵工业, 1994, 0(4): 44-48 |
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| 作者姓名： | 刘红 潘红春 |
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| 作者单位： | 四川轻化工学院轻工系 |
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| 摘 要： | 利用正交试验法对钛氯活化纤维素固定核酸酶Ｐ_１的工艺条件进行优化，获得了较优的固定化工艺条件，酶活力回收提高到８４．５％，降解酵母ＲＮＡ的５＇－核苷酸生成率平均可达７４．２％。
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| 关 键 词： | 正交试验，钛氯纤维素，核酸酶P_1，固定化，5’－核苷酸 |
Studies on the Immobilization of Nuclease P_1 |
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| Liu Hong, Pan Hongchun, Ding Hong, Wang Xiaolan. Studies on the Immobilization of Nuclease P_1[J]. Food and Fermentation Industries, 1994, 0(4): 44-48 |
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| Authors: | Liu Hong Pan Hongchun Ding Hong Wang Xiaolan |
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| Abstract: | The technology conditions of nuclease P, immobilized with TiCl_3-cellulose is selected by orthogonal experiments. A better immobilized technology conditions is gained. The recovery of activity of nuclease P_1 is raied to 84. 5%. The yield of 5’-nuleotides from hydrolyzing yeast RNA averages 74.2%. |
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| Keywords: | Orthogonal experiments TiCl_3-cellulose Nuclease P_1 Immobilization 5’-nucleotide |
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