栀子对重症急性胰腺炎胰腺细胞结构和功能的影响及其与NO、内毒素、氧自由基的关系

目的探讨栀子对重症急性胰腺炎(SAP)胰腺细胞结构和功能的影响及其与一氧化氮(NO)、内毒素、氧自由基(OFR)的关系。方法经胆胰管逆行注射1.5%去氧胆酸钠建立SAP模型,给予栀子治疗,观察血中淀粉酶、内毒素、一氧化氮(NO)、过氧化脂质(LPO)以及胰腺细胞线粒体琥珀酸脱氢酶(SDH)活性、溶酶体酸性磷酸酶(ACP)释放率、微粒体细胞色素P450的变化,并进行相关分析及胰腺组织病理学检查。结果与对照组比较,模型组血中淀粉酶、NO、内毒素、LPO以及溶酶体ACP释放率明显升高(P<0.05),线粒体SDH活性和微粒体细胞色素P450则显著降低(P<0.05),光镜下胰腺组织病理损害明显;栀子治疗后上述各检测指标均较模型组明显改善(P<0.05);相关分析显示淀粉酶、NO、内毒素、LPO与溶酶体ACP释放率呈正相关,与线粒体SDH和微粒体细胞色素P450呈明显负相关。结论NO、内毒素、OFR破坏胰腺细胞的结构和功能。栀子能减轻三者的损伤,保护胰腺细胞。

The effects of gardenia jasminoides ellis on the structure and function of pancreatic cell in severe acute pancreatitis and its relationships with NO,eendotoxin and oxygen derived free radical

Objective: To investigate the effects of gardenia jasminoides ellis (GJE) on the structure and function of pancreatic cell in rats with severe acute pancreatitis (SAP) and its relationships with nitric oxide (NO) , endotoxin , oxygen derived free radical (OFR) .Methods: SAP models were induced by retrograde injection of 1.5% sodium deoxycholate and treated by GJE. The changes of amylase,NO,endotoxin,lipid peroxide(LPO),succinic dehydrogenase (SDH) in mitochondria,acid phosphatase(ACP) in lysosome and cytochrome P_ 450 in microsome were measured, and the correlations between them were analysed. The changes of pancreatic histology were examined by light microscope. Results: Compared with control group , amylase ,NO,endotoxin, LPO and the releasing rate of ACP in lysosome in SAP group were significantly higher; SDH in mitochondria and cytochrome P_ 450 in microsome were lower; the damage to pancreatic tissue was increased in severity with light microscope. The above-mentioned indexes of GJE group were obviously ameliorated in comparison with SAP group. The correlation analysis indicated that amylase, NO , endotoxin and LPO were positively correlated with the releasing rate of ACP in lysosome ,negatively with SDH in mitochondria and cytochrome P_ 450 in microsome.Conclusion: NO, endotoxin and OFR damage the structure and function of pancreatic cell. GJE can alleviate their damage and protect pancreatic cell.