鸡胚肾（小管）发生中相关活性物质的表达及调控意义

目的观察表皮生长因子受体(EGFR)、转化生长因子-β(TGF-β)和水通道蛋白-2(AQP-2)及Bax蛋白在鸡胚肾(小管)发生过程中的表达,探讨它们的生物活性作用及相互之间的调控意义。方法应用免疫组织化学染色和体视学半定量方法,检测EGFR、TGF-β(β1、β2、β3)、AQP-2及Bax蛋白在10~46期鸡胚共36例肾组织发生中的表达,用IPP专业图像分析软件对其表达强度进行定量分析。结果第26~46期鸡胚肾的近端小管上皮细胞呈EGFR免疫反应阳性;第26~37期中肾的近端小管上皮细胞和远端小管上皮细胞呈TGF-β免疫反应阳性,第33~40期后肾的近端小管上皮细胞和远端小管上皮细胞呈TGF-β免疫反应阳性,第44~46期近端小管上皮细胞呈TGF-β免疫反应阳性,远端小管上皮细胞呈TGF-β免疫反应阴性;第26~46期鸡胚肾的集合小管上皮细胞呈AQP-2免疫反应阳性;第26~37期中肾近端小管和远端小管上皮细胞呈Bax反应阳性,第33~46期后肾近端小管上皮细胞呈Bax免疫反应阳性。图像分析显示,在鸡胚肾发生过程中,EGFR、TGF-β(β1、β2、β3)和Bax蛋白的阳性反应呈现先增强后减弱的变化,AQP-2阳性反应呈持续增强的趋势。结论鸡的中肾具有排泄功能,中肾肾小管的退化可能与TGF-β和Bax蛋白的大量表达有关。EGFR、TGF-β、AQP-2和Bax蛋白的协同表达调控着后肾肾小管和集合小管的发生及分化成熟。在肾脏发育过程中AQP-2可能介导胚胎发育过程中水平衡的稳定。

EXPRESSION AND REGULATORY FUNCTION OF INTERRELATED POLYPEPTIDES DURING DEVELOPMENT OF RENAL TUBULES IN CHICK EMBRYO

Objective To observe the expression of EGFR，TGF-β，AQP-2 and Bax during development of renal tubules in chick embryo，and explore the role and regulating significance of them. Methods The expression of EGFR，TGF-β，AQP-2 and Bax were observed by immunohistochemistry combined with stereological methods in 36 chicks kidney at 10th-46th incubation stages. The expression intensity of them was analyzed by IPP software. Results The epithelial cells of proximal tubules in chick embryo appeared EGFR immunoreactivity at 26th-46th stages；At 26th-37th stages the epithelial cells of proximal tubules and distal tubules of mesonephros appeared TGF-β immunoreactivity，the epithelial cells of proximal tubules and distal tubules of metanephros appeared TGF-β immunoreactivity at 33rd-40th stages，at 40th-46th stages the epithelial cells of proximal tubules of metanephros appeared TGF-β immunoreactivity but the epithelial cells of distal tubules did not appeare TGF-β immunoreactivity；The epithelial cells of collecting tubules in chick embryo appeared AQP-2 immunoreactivity at 26th-46th stages；The epithelial cells of proximal tubules and distal tubules of mesonephros appeared Bax immunoreactivity at 26th-37th stages，the epithelial cells of proximal tubules of metanephros appeared Bax immunoreactivity at 33rd-46th stages. Images showed the expressions of EGFR，TGF-β（βSUB>1/SUB>，β SUB>2/SUB>，βSUB>3/SUB>）and Bax first displayed up then down，but expression of AQP-2 always displayed up during develop