幽门螺杆菌感染的成石胆汁促进人胆管癌细胞增殖的研究

目的探讨幽门螺杆菌感染的成石胆汁对人胆管癌细胞QBC939生长的影响，阐明幽门螺杆菌感染与胆管癌发生、发展的内在关系。方法应用噻唑蓝比色法检测20份幽门螺杆菌阳性胆管结石患者胆汁、20份幽门螺杆菌阴性胆管结石患者胆汁以及20份正常胆汁对QBC939细胞增殖的影响，运用流式细胞仪测定细胞周期与凋亡。结果与幽门螺杆菌阴性胆管结石患者胆汁和正常胆汁比较，幽门螺杆菌感染的成石胆汁明显促进QBC939细胞增殖（P〈0.05），用幽门螺杆菌感染的成石胆汁处理48h的QBC939细胞增殖指数显著上升（P〈0.01），S期细胞比例明显增高（P〈0.05），G0／G1期细胞比例明显降低（P〈0．05）。结论幽门螺杆菌感染的成石胆汁具有强大的潜在促增殖活性，胆道幽门螺杆菌感染与胆管癌的发生、发展关系密切。

Experimental study on the proliferative activity for a cholangiocarcinoma cell line promoted by bile from patients with choledocholithiasis infected by helicobacter pylori

Objective To explore the effects of bile from patients with choledocholithiasis infected by helicobacter pylori on the growth of human cholangiocarcinoma cell line QBC939 and elucidate the potential relation between infection of helicobacter pylori and cholangiocarcinoma.Methods By using methabenzthiazuron(MTT) assay,the effects of choledocholithiasis bile with helicobacter pylori(PCB),choledocholithiasis bile without helicobacter pylori(NCB) or normal bile(NB) on the proliferative activity of QBC939 were measured.Cell cycle and apoptosis were detected by flow cytometry.Results As compared with NB,PCB significantly promoted the proliferation of QBC939 cells.QBC939 proliferative index was increased obviously after treated with 1% PCB for 48 h.The percentage of S phase was increased markedly,while the percentage of G_0/G_1 phase decreased remarkably.Conclusion PCB can greatly promote the proliferation of human cholangiocarcinoma QBC939 cells,so we can predict that the infection of helicobacter pylori had a good relation with cholangiocarcinoma.