携带Fas基因重组腺病毒治疗瘢痕疙瘩的体内实验研究

目的 研究携带人Fas基因的两种重组腺病毒对瘢痕疙瘩的体内治疗效果。方法 构建瘢痕疙瘩裸鼠模型,应用携带Fas基因的常规腺病毒Ad—Fas（T）和细菌内重组腺病毒Ad—Fas（B）注射及Fas单克隆抗体（FasMcab）辅助对植入裸鼠皮下的瘢痕疙瘩组织进行治疗。通过大体观察、常规病理及电镜观察检测瘢痕疙瘩组织的变化。结果 单纯使用腺病毒注射的瘢痕疙瘩组织块体积仅轻度缩小。前期注射Ad—Fas（B）或Ad—Fas（T）后,应用FasMcab作为后续治疗,瘢痕疙瘩组织块均明显缩小,HE染色证实瘢痕疙瘩组织结构遭到破坏,电镜观察发现细胞凋亡证据。结论 重组腺病毒Ad—Fas（B）及Ad—Fas（T）的瘢痕疙瘩基因治疗效果令人满意。为瘢痕疙瘩的治疗提供了新途径。

Experimental gene therapy of keloid in vivo using recombinant adenovirus carrying Fas gene

OBJECTIVE: To observe the gene therapeutic effects of two kinds of prepared recombinant adenovirus coding for Fas gene on keloid in nude mouse model. METHODS: Keloid tissue was transplanted into athymic nude mice subcutaneously to establish the animal model of keloid. Forty-five days after the model had been established, the recombinant adenovirus vectors carrying Fas gene [Ad-Fas (B) or Ad-Fas (T)] or empty vector were injected into the keloid tissues. One day after that, anti-Fas monoclonal antibody (FasMcab) was injected in the keloid tissues. And 7 days after, the animals were sacrificed and changes of the keloid tissues were observed generally and by pathological and electronic microscopic examinations. RESULTS: The keloid tissues shrunk slightly in the animals injected adenovirus only. The keloid tissues diminished significantly in the animals after injection of Ad-Fas (T) or Ad-Fas (B) followed by FasMcab. Destroyed structure of keloid tissue was found with HE stain. Obvious apoptosis was testified with the electronic microscope. CONCLUSIONS: Gene therapy of keloid in vivo with recombinant adenovirus carrying Fas gene is effective, and it maybe a potential therapy for keloid.