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Effect of diacetyl piquerol on H+-ATPase activity of microsomes fromIpomoea purpurea
Authors:R Cruz Ortega  A L Anaya  M Gavilanes-Ruiz  S Sanchez Nieto  M Jimenez Estrada
Affiliation:(1) Instituto de Fisiología Celular, Universidad National Autónoma de Méxco, Apartado Postal 70-600, 04510 México, D.F., Mexico;(2) Departamento de Bioquítmica, Facultad de Qmímica, Universidad National Autónoma de Méxco, Apartado Postal 70-600, 04510 México, D.F., Mexico;(3) Instituto de Quimica, Universidad National Autónoma de Méxco, Apartado Postal 70-600, 04510 México, D.F., Mexico
Abstract:The effect of an allelopathic compound, diacetyl-piquerol on the H+ -ATPase activity of the microsomal fraction from the radicles of a common weedIpomoea purpurea was studied. The diacetyl-piquerol inhibited the germination and radicle growth fromI. purpurea; the radicle growth was increasingly inhibited (10% to 100%) as piquerol concentrations were raised (10 mgrM to 1000 mgrM). The H+-ATPase activity was inhibited (48%) by 500 mgrM diacetyl-piquerol, and this inhibition was higher in plasma membrane ATPase (67.2%) than in tonoplast membrane ATPase (31.4%). Additional studies of the precise physiological mechanisms of interference caused by allelopathic compounds are needed.This study was sponsored by Project PCECCCNA-050954 of Consejo Nacional de Ciencia y Tecnología (CONACyT).
Keywords:Piquerol  Piqueria trinervia  modes of action  allelopathy  allelochemicals  microsomes  Ipomoea purpurea  plasmalemma  tonoplast  germination  H+-ATPase  proton pumps
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