| 抗莱氏支原体单克隆抗体的研制 |
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| 引用本文： | 曾星,杨明,刘力,夏庆苏,陈兆聪.抗莱氏支原体单克隆抗体的研制[J].华中科技大学学报(医学版),1995(3). |
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| 作者姓名： | 曾星 杨明 刘力 夏庆苏 陈兆聪 |
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| 作者单位： | 同济医科大学实验医学研究中心分子生物学研究室，武汉市第二医院外科 |
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| 摘 要： | 通过细胞融合技术，筛选出ＭＡＬ－１和ＭＡＬ－２两株抗莱氏支原体（Ａ．Ｌａｉｄｌａｗｉｉ，Ａ．Ｌ）单克隆抗体（单抗）。通过ＳＤＳ－ＰＡＧＥ和Ｗｅｓｔｅｒｎ印迹法测得单抗相对应的抗原分子量均为６７．４６ｋｕ。用间接免疫荧光法（ＩＦＡ）检测，单抗与细胞培养中另外３种常见污染的支原体和呼吸道肺炎支原体无交叉反应。将单抗用于检测支原体污染的细胞培养物，结果表明，此单抗能特异性地检出莱氏支原体。故可用其推断支原体污染途径，便于制定防治措施。
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| 关 键 词： | 莱氏支原体 抗体．单克隆 细胞污染 |
A Study on Monoclonal Antibodies against A.Laidlawii Mycoplasma |
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| Zeng Xing,Yang Ming,Liu Li,et al.A Study on Monoclonal Antibodies against A.Laidlawii Mycoplasma[J].Journal of Huazhong University of Science and Technology(Health Sciences),1995(3). |
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| Authors: | Zeng Xing Yang Ming Liu Li |
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| Abstract: | By using cell fusion technique,two monoclonal antibodies,MAL-1 and MAL-2,against A.Laidlawii(A.L) were produced.The molecular weight of the A.L antigens was 67.46ku as determined by SDS-PAGE and Western blot.These two MAbs showed no cross-reaction to other four Mycoplasmas,M.Hyorhinis,M.Orale,M.Ariginini and M.Pneumoniae,as tested by indirect fluorescence assay(IFA).Our study showed that these antibodies could be used for specific detection of A.Laidlawii. |
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| Keywords: | mycoplasma antibody monoclonal cell contamination |
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