| 高效液相色谱荧光检测法同时测定婴幼儿食用鱼粉中环丙沙星和恩诺沙星残留量 |
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| 引用本文： | 王红青,姜荷,屠海云,黄建萍,林伟杰. 高效液相色谱荧光检测法同时测定婴幼儿食用鱼粉中环丙沙星和恩诺沙星残留量[J]. 中国卫生检验杂志, 2011, 0(1): 50-52 |
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| 作者姓名： | 王红青 姜荷 屠海云 黄建萍 林伟杰 |
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| 作者单位： | 杭州质量技术监督检测院; |
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| 摘 要： | 目的:建立用高效液相色谱荧光检测器同时测定婴幼儿食用鱼粉中环丙沙星和恩诺沙星残留的方法。方法:试样经水复原后,由5%乙酸乙腈提取,正己烷脱脂,以0.05 mol/L磷酸三乙胺缓冲液-乙腈(体积比为85∶15)为流动相洗脱,荧光检测器定性定量。结果:该方法对测定的两种药物的检出限均为5μg/kg,定量限均为10μg/kg。两种药物在5μg/L~80μg/L范围内线性关系良好,相关系数均大于0.99。在添加水平分别为10μg/kg、20μg/kg和80μg/kg时,两种药物的加标回收率为80.3%~92.6%,相对标准偏差(RSD)为1.2%~3.8%。结论:方法操作简单、重现性好、灵敏度高、分析时间短,适用于鱼粉中环丙沙星和恩诺沙星残留的确证检测。
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| 关 键 词： | 高效液相色谱 荧光检测 婴幼儿食用鱼粉 环丙沙星 恩诺沙星 |
Simultaneous determination of ciprofloxacin and enrofloxacin residues in fish meal for children by high performance liquid chromatography with fluorescence detector |
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| WANG Hong-qing,JIANG He,TU Hai-yun,HUANG Jian-ping,LIN Wei-jie. Simultaneous determination of ciprofloxacin and enrofloxacin residues in fish meal for children by high performance liquid chromatography with fluorescence detector[J]. Chinses Journal of Health Laboratory Technology, 2011, 0(1): 50-52 |
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| Authors: | WANG Hong-qing JIANG He TU Hai-yun HUANG Jian-ping LIN Wei-jie |
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| Affiliation: | WANG Hong-qing,JIANG He,TU Hai-yun,HUANG Jian-ping,LIN Wei-jie(Hangzhou Institute of Test and Calibration for Quality and Technology Supervision,Hangzhou 310019,China) |
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| Abstract: | |
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| Keywords: | High performance liquid chromatography Fluorescence detection Fish meal Ciprofloxacin Enrofloxacin |
| 本文献已被 CNKI 等数据库收录！ |
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