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| HPLC法同时测定胃痛欣颗粒剂中大黄酸、大黄素、大黄酚的含量 | |
| 引用本文： | 贾英,董娥,杨松,包文芳.HPLC法同时测定胃痛欣颗粒剂中大黄酸、大黄素、大黄酚的含量[J].沈阳药科大学学报,2003,20(2):123-125. |
| 作者姓名： | 贾英 董娥 杨松 包文芳 |
| 作者单位： | 1. 沈阳药科大学中药学院,辽宁沈阳,110016 2. 大连市金州区第一人民医院,辽宁大连,116100 3. 沈阳军区药品检验所,辽宁沈阳,110026 |
| 摘 要： | 目的建立胃痛欣颗粒剂中大黄酸、大黄素、大黄酚的含量测定方法。方法采用高效液相色谱法 ,AccusilC1 8(4 6mm× 2 5 0mm ,1 0 μm)柱 ,流动相为 :甲醇 水 冰醋酸 (80∶2 0∶1 ,V∶V∶V) ,检测波长为 2 5 4nm。结果大黄酸、大黄素、大黄酚分别在 0 0 3～ 0 4 8μg ,0 0 2 5～ 0 4 0 μg ,0 0 2 5～0 4 0 μg内与峰面积呈线性关系 ,平均回收率 (n =3 )分别为 98 3 % (RSD =1 4 3 % ) ,97 6% (RSD=1 84 ) ,98 7% (RSD =0 5 7% )。结论可用于大黄药材及其复方制剂的质量控制 |
| 关 键 词： | 高效液相色谱法 胃痛欣 大黄酸 大黄素 大黄酚 |
| 文章编号： | 1006-2858(2003)02-0123-03 |
| 修稿时间： | 2002年5月10日 |
Simutaneous determination of the contents of rhein,emodin and chrysophanol in Weitongxin Granules by HPLC |
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| JIA Ying ,DONG E ,YANG Song ,BAO Wen-fang.Simutaneous determination of the contents of rhein,emodin and chrysophanol in Weitongxin Granules by HPLC[J].Journal of Shenyang Pharmaceutical University,2003,20(2):123-125. | |
| Authors: | JIA Ying DONG E YANG Song BAO Wen-fang |
| Affiliation: | JIA Ying 1,DONG E 2,YANG Song 3,BAO Wen-fang 1 |
| Abstract: | Objective To develop a HPLC method for the determination of the contents of rhein,emodin and chrysophanol in the Weitonxin Granules.Methods The measurement conditions used were as follows: Accusil C 18( 4.6 mm× 250 mm)column; mobile phase: methanol-H 2O-acetic acid(80∶20∶1,V∶V∶V); flow rate: 1 mL/min; wavelength for rhein, emodin and chrysophanol showed good linear relation. The recovery rates for the rhein, emodin and chrysophanol were 98.3%(RSD=1.43%),97.6%(RSD=1.84%),98.7%(RSD=0.57%)respectively.The conditions of extraction and detemination were optimized.Conclusions The procedure is simple and rapid.The method can be used for evaluation of the crude drugs and their preparations. |
| Keywords: | HPLC weitongxin granules rhein emodin chrysophanol |
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