纳米硒对大鼠急性胃粘膜损伤的保护作用

目的研究不同剂量纳米硒对大鼠急性胃粘膜损伤是否有保护作用。方法将50只健康Wister大鼠随机分为5组:正常对照组、模型对照组、纳米硒低、中、高剂量组,含纳米硒剂量分别为4.17、8.33和16.67μg/kg,用无水乙醇诱导大鼠急性胃粘膜损伤(AGML),然后进行胃粘膜组织病理学检查,分别测定各组大鼠胃粘膜损伤指数(UI)、血清谷胱甘肽过氧化物酶(GSH-Px)活性、丙二醛(MDA)含量。结果与模型对照组比较,纳米硒各剂量组的损伤指数显著降低(P<0.01),且呈剂量依赖性,血清中GSH-Px活性显著升高(P<0.01),MDA含量下降(P<0.05,P<0.01),粘膜损伤程度明显减轻。结论纳米硒对无水乙醇诱导的急性胃黏膜损伤有明显的保护作用,其机制可能与抗氧化作用有关。

Protective effects of nanometer selenium on acute gastric mucosal lesion in rats

Objective To study the protective effects of nanometer selenium on acute gastric mucosal lesion in rats.Methods 50 healthy Wister rats were divided into five groups randomly:normal group,model group,and three experimental groups(4.17,8.33 and 16.67mg/kg).The lesion of gastric mucosal was established by giving absolute alcohol after giving nanometer selenium of different dosage to rats except normal group,examined histopathology of gastric mucosa,then measured the lesion index(UI)of rats in normal group,model group and three experimental groups.The activity of glutathione peroxidase(GSH-Px) and malondialdehyde(MDA) content in blood were determined.Results Gastric mucosal lesion index of all the three experimental groups was obviously reduced compared with the control groups(P<0.01) and existed dose dependent.The nanometer selenium elevated GSH-Px activity(P<0.01) but reduced MDA content(P<0.05,P<0.01).In addition,the extent of mucosal lesion of experimental groups was significantly less than that of experimental control.Conclusion Nanometer selenium can significantly prevent absolute ethanol-induced acute gastric mucosal lesion.The mechanism may be related to the antioxidation of the powder.