低频超声联合微泡及脑源性神经营养因子预防治疗大鼠缺血性卒中

目的 探讨脑源性神经营养因子（BDNF）在超声联合微泡介导下对急性缺血性脑卒中大鼠模型的预防性治疗作用。方法 将27只健康SD大鼠随机分为假手术组、BDNF组和BDNF超声组，每组9只，建立大脑中动脉梗阻（MCAO）模型，评价术后24 h脑组织中BDNF含量，并于术后第1、3和7天进行MR检查以及行为学评价。结果 BDNF超声组脑组织中的BDNF含量明显高于BDNF组及假手术组（P均<0.05）。BDNF超声组脑梗死面积随时间延长而逐渐缩小，第7天时，梗死面积明显小于BDNF组。BDNF超声组和BDNF组术后第7天行为学评价差异有统计学意义（P<0.05）。结论 超声介导下BDNF可增强对急性缺血性脑卒中大鼠模型的预防性治疗作用，并可明显减小梗死灶，改善行为学表现。

Low frequency ultrasound combined with microbubbles and brain-derived neurotrophic factor drugs in prevention of ischemic stroke in rats

Objective To explore the preventive therapeutic effects of brain-derived neurotrophic factor (BDNF) on acute ischemic stroke in rats under ultrasound combined with microbubbles.Methods Twenty-seven healthy SD rats were randomly divided into sham-operation group (n=9), BDNF group (n=9) and BDNF plus ultrasound group (n=9), then middle cerebral artery occlusion (MCAO) models were established. The concentration of BDNF in targeted brain tissue was measured at 24 h after operation. MRI examination and behavioral assessment were performed at 1, 3 and 7 days after operation.Results The concentration of BDNF in BDNF plus ultrasound group was significantly higher than that in BDNF group and sham operation group (all P<0.05). The infarct size in BDNF group gradually reduced with time increase, and the infarct size was significantly smaller than that of BDNF group at the 7th day. The behavioral assessment had a significant statistical difference between BDNF plus ultrasound group and BDNF group at the 7th day after the operation (P<0.05).Conclusion The preventive therapeutic effect of BDNF could be enhanced by ultrasound in the rat model with acute ischemic stroke, which can significantly reduce the size of the infarct and improve the behavior of the rats.