黑素瘤抗原-1基因在非小细胞肺癌中的表达

目的:检测黑素瘤抗原-1(MAGE-1) mRNA及其编码的肿瘤抗原在非小细胞肺癌(NSCLC)中的表达,探讨以该抗原作为疫苗对NSCLC患者进行免疫治疗的可能性.方法:用RT-PCR方法和流式细胞术(FCM)对3种人肺癌细胞系、56例NSCLC标本和相邻正常肺组织标本MAGE-1 mRNA及蛋白的表达情况进行检测.结果:MAGE-1 mRNA及蛋白在3种人肺癌细胞系均表达;56例NSCLC标本中,27例表达MAGE-1 mRNA,22例表达MAGE-1蛋白,而在相邻正常肺组织中均不表达.结论:MAGE-1 mRNA及蛋白在NSCLC中具有较高的表达频率,提示该抗原有望成为对NSCLC患者进行免疫治疗的适宜靶点.

Expression of melanoma antigen-1 gene in non-small cell lung cancer

Objective: To determine the expression of melanoma antigen-1(MAGE-1) mRNA and its encoding tumor antigen in non-small cell lung cancer (NSCLC) and to explore the possibility of using it as a vaccine for immunotherapy in NSCLC patients. Methods: The expression of MAGE-1 mRNA and protein in 3 human lung cancer cell lines and 56 NSCLC samples together with the adjacent normal lung samples was examined by RT-PCR method and flow cytometry (FCM). Results: All 3 human lung cancer cell lines expressed MAGE-1 mRNA and protein. Of the 56 NSCLC samples, 27 expressed MAGE-1 mRNA and 22 expressed MAGE-1 protein, whereas none of the adjacent normal lung samples expressed MAGE-1 mRNA or protein. Conclusion: MAGE-1 mRNA and protein are expressed at a high frequency in NSCLC samples, and this antigen may be the suitable target for immunotherapy for NSCLC patients.