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| RNAi对白血病细胞mdr-1基因和多药耐药表型的影响 | |
| 引用本文： | 彭智,冯文莉,肖志坚,刘澎,王一,韩忠朝. RNAi对白血病细胞mdr-1基因和多药耐药表型的影响[J]. 肿瘤, 2006, 26(12): 1074-1077 |
| 作者姓名： | 彭智 冯文莉 肖志坚 刘澎 王一 韩忠朝 |
| 作者单位： | 1. 广州中医药大学第一附属医院检验科 2. 重庆医科大学医学检验系临床血液学教研室,教育部临床检验诊断学重点实验室,重庆,400016 3. 中国医学科学院中国协和医科大学天津血液学研究所,天津,300020 |
| 摘 要： | 目的:探讨RNA干扰技术(RNAi)对慢性粒细胞白血病急变细胞系K562/AO2细胞mdr1基因的抑制和耐药表型的逆转作用。方法:选择合成封闭mdr-1基因的小干扰序列(si-MDR1),以1个碱基突变的si-MDR1-mut为对照序列,在脂质体介导下转染至K562/AO2细胞系。RT-PCR和Western blot检测mdr1 mRNA及P-gp蛋白水平,流式细胞术分析细胞内柔红霉素(daunorubicin,DNR)积累量,并以四甲基唑蓝快速比色法(MTT)反映K562/AO2对阿霉素、长春新碱、足叶乙甙药物敏感性的变化。结果:实验证实该序列能高效封闭K562/AO2细胞内mdr-1基因表达,增加细胞内化疗药物DNR积累量,增强K562/AO2细胞对阿霉素、长春新碱、足叶乙甙的敏感性。结论:RNAi可以通过抑制mdr1基因表达,逆转K562/AO2细胞耐药表型。 |
| 关 键 词： | 白血病 RNA,小干扰 基因,mdr-1 K562细胞 |
| 文章编号： | 1000-7431（2006）12-1074-04 |
| 收稿时间： | 2005-12-18 |
| 修稿时间： | 2006-02-08 |
Effect of RNA interference on expression of mdr1 gene and multidrug resistence phenotype of leukemia cells |
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| PENG Zhi,FENG Wen-li,XIAO Zhi-jian,LIU Peng,WANG Yi,HAN Zhong-chao. Effect of RNA interference on expression of mdr1 gene and multidrug resistence phenotype of leukemia cells[J]. Tumor, 2006, 26(12): 1074-1077 | |
| Authors: | PENG Zhi FENG Wen-li XIAO Zhi-jian LIU Peng WANG Yi HAN Zhong-chao |
| Affiliation: | 1. Department of Clinical Hematology, Chongqing University of Medical Sciences, Key Laboratory of Clinical Medical Diagnostics of Education Ministry,Chongqing 400016, China; 2. Institute of Hematology, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China |
| Abstract: | Objective: To study the effect of RNA interference on expression of mdr1 gene and multidrug resistance phenotype in chronic myeloid leukemia-blastic crisis K562/AO2 cells.Methods:MDR1-targeted small interfering RNA(si-MDR1) was selectively synthesized.Si-MDR1 with a single-base-pair mutation was used as control(si-MDR1-mut).They were transferred into K562/AO2 cells mediated by liposome oligofectamine.The levels of mdr1 mRNA and P-gp protein were detected by RT-PCR and Western blotting,respectively.Daunorubicin(DNR) accumulation was analyzed by flow cytometry analysis.Sensitivities of K562/AO2 cells to adriamycin(ADM),vincristine(VCR),and etoposide(VP-16) were detected by MTT assay.Results:Mdr1-targeted small interfering RNA duplex effectively interfered with the expression of mdr1 and inhibited P-gp protein expression in drug-resistant leukemia K562/AO2 cells,increased intracellular DNR accumulation,and enhanced chemosensitivity of K562/AO2 cells to ADM,VCR,and VP-16.Conclusion: RNA interference reversed multidrug resistance phenotype of K562/AO2 cells via inhibition of mdr-1 gene expression. |
| Keywords: | Leukemia RNA small interfering Genes mdr-1 K562 cells |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
