桑树桑黄总三萜提取工艺优化及其降血脂、抗氧化活性研究

选用超声波法提取桑树桑黄子实体中的三萜类化合物,在单因素试验的基础上采用响应面法优选最佳方案,并对其体外降血脂和抗氧化活性进行了测定.结果表明,超声波法提取桑树桑黄总三萜的最优条件为:提取时间49 min、乙醇浓度为80％、提取温度为61℃,料液比为1∶22(g/mL),总三萜得率可达12.32％±0.17％.质量浓度...

Optimization of Extraction Technology of Total Triterpenoids from Inonotus sanghuang and Their Hypolipidemic and Antioxidant Activities

The ultrasonic-assisted extraction process of total triterpenoids from Inonotus sanghuang was employed in this paper.Based on the single factor experiments,the response surface methodology(RSM)was used to determine the optimal extraction condition,and its hypolipidemic and antioxidant activities were primarily evaluated.The results of optimum extraction conditions were as follow:Extraction time 49 min,ethanol concentration 80%,ultrasonic temperature 61℃,ratio of solid to liquid 1:22(g/mL).Under these conditions,the yield of total triterpenoids was 12.32%±0.17%.The binding ability of 80 mg/mL triterpenoids to sodium glycylcholate,sodium taurocholate and sodium cholate in vitro was 50.93%,52.14%and 43.06%of that of colenamine in the same dose,which indicated that the total triterpenoids of Inonotus sanghuang had the activity of lowering blood lipids in vitro.The iron ions reduction results showed that the total triterpenoids had certain reducing power.The IC50 values of scavenging capacities of DPPH and ABTS free radical was 0.304 and 0.520 mg/mL,respectively,which showed it possessed good antioxidants in vitro.