2-苯基-4-对氯苯基-1,5-苯并硫氮杂-α-(丁二酰亚胺基)-β-内酰胺的高效液相色谱法分析

采用Hypersil-ODS2(4.6mm×250mm,5μm)色谱柱,乙腈-水(70∶30)流动相,242nm检测波长,建立了测定2-苯基-4-对氯苯基-1,5-苯并硫氮杂-α-(丁二酰亚胺基)-β-内酰胺的高效液相色谱法。该法线性范为0～0.1mg/mL,回归方程为A=1.63×108c-2.86×105,相关系数r=0.9994,相对标准偏差为0.33%(c=0.06mg/mL,n=5),检出限为0.08μg/mL。平均回收率在97.2%～102.4%之间。该法重现性好,灵敏度高,简单,快速,准确。

Determination of 2-Phenyl-4-(4-chloridebenzoyl)-1,5-benzothiazepion-α-succinimide-β-lactam by High Performance Liquid Chromatography

HPLC method was used for determination of 2-phenyl-4-(4-chloridebenzoyl)-1,5-benzothiazepion-α-succinimide-β-lactam. The expevimeat was carried out on a hypersil-ODS_2(4.6 mm×250 mm, 5 μm)column at 242 nm with a acetonitrile-water mixture (70∶30(v/v)) as the mobile phase. A good linear relationship exists in the range of 0～0.1 mg/mL (r=0.9994). The average recoveries are 97.2%～102.4% with RSD=0.33% (c=0.06 mg/mL,n=5),This method is simple and rapid, and provides high sensitivity, accuracy and reproducibility.