应用人脐带血单个核细胞体外同时表达EB病毒和巨细胞病毒蛋白抗原

目的：拟建立一种能预防和治疗移植后病毒感染的抗原呈递细胞（ａｎｔｉｇｅｎ－ｐｒｅｓｅｎｔｉｎｇ ｃｅｌｌ，ＡＰＣ）。方法：利用人的脐带血单个核细胞（ＣＢ－ｄｅｒｉｖｅｄ ｍｏｎｏｎｕｃｌｅａｒ ｃｅｌｌｓ，ＣＢＭＣ），用ＥＢ病毒（Ｅｐｓｉｅｉｎ－Ｂａｒｒ ｖｉｒｕｓ，ＥＢＶ）感染转化成Ｂ淋巴母细胞细胞系（Ｂ－ｌｙｍｐｈｏｂｌａｓｔｏｉｄ ｃｅｌｌ ｌｉｎｅｓ，ＢＬＣＬ），再用编码有ＣＭＶ．ｐｐ６５

Human umbilical cord blood simultaneously presenting cytomegalovirus pp65 and Epstein-Barr virus antigens in vitro

Objective: To establish an antigen presenting cells (APC) for treatment and prevention of cytomegalovirus (CMV) and Epstein Barr virus (EBV) infection after transplantation. Methods: Using human umbilical cord blood derived mononuclear cell (CBMC), we engineered EBV transformed B lymphoblastoid cell lines (BLCL). BLCL were transduced with a recombinant retrovirus encoding CMV.pp65. CMV.pp65 and EBV antigen simultaneous expression in BLCL were confirmed by Western blot analysis and immunocytochemistry. Result: We obtained the antigen presenting cell line BLCL pp65, which can simultaneously express CMV.pp65 and EBV antigen. Conclusion: The present method can establish a cell line which can simultaneously express CMV.pp65 and EBV antigen.