| PAK株与PA103株绿脓杆菌外毒素结构基因间的差异及其对功能的影响 |
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| 引用本文： | 高基民,林来兴妹,黄洪莲,王小宁,徐羚飞,郑仲承,刘新垣.PAK株与PA103株绿脓杆菌外毒素结构基因间的差异及其对功能的影响[J].中国生物化学与分子生物学报,1996,12(1):31-35. |
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| 作者姓名： | 高基民 林来兴妹 黄洪莲 王小宁 徐羚飞 郑仲承 刘新垣 |
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| 作者单位： | 第一军医大学免疫学教研室，中科院上海生物化学研究所 |
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| 基金项目： | “86．3”、国家自然科学基金，全军青年科学基金，广东省青年科学基金 |
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| 摘 要： | 对ＰＡＫ株的绿脓杆菌外毒素结构基因进行全长核苷酸顺序测定时发现：来源于ＰＡ１０３株和ＰＡＫ株的ＰＥ基因一级结构间存在差异，其中Ｔｈｒ~（１７９）（ＡＣＣ）→Ａｌａ~（１７９）（ＧＣＣ）、Ｓｅｒ~（５１５）（ＡＧＣ）→Ｇｌｙ~（５１５）（ＧＧＣ），并有１１个同义突变，但变异并不影响白细胞介素２－绿脓杆菌外毒素融合蛋白的ＡＤＰ－核糖基化活性及其细胞毒活性。
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| 关 键 词： | 绿脓杆菌外毒素 核苷酸顺序 突变 ADP－核糖基化活性 细胞毒活性 |
| 收稿时间： | 1996-02-20 |
Differences between Structural Gene of Pseudomonas Exotoxin from PAK Strain and That from PA103 Strain and Their Effects on its Function |
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| Gao Ji-Min,Lin Lai-Xing-Mei,Huang Hong-Lian,Wang Xiao-Ning.Differences between Structural Gene of Pseudomonas Exotoxin from PAK Strain and That from PA103 Strain and Their Effects on its Function[J].Chinese Journal of Biochemistry and Molecular Biology,1996,12(1):31-35. |
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| Authors: | Gao Ji-Min Lin Lai-Xing-Mei Huang Hong-Lian Wang Xiao-Ning |
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| Affiliation: | (The First Military Medical University, Guangzhou 510515) Xu Ling-Fe; Zheng Zhong-Cheng; Liu Xin-Yuan(Shanghai Institute of Biochemistry, Academia Sinica, Shanghai 200031 |
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| Abstract: | The nucleotide sequence of structural gene of Pseudomonas exotoxin from PAK strain was determined, and its differences from that of PAK103 were first discovered. Besides several consense alteration, there were two amino acid differences such as Thr 179 (ACC) → Ala 179 (GCC) and Sersis (AGC)→ Gly 515 (GGC), but these differences had no effects on ADP-ribosylation and the cytotoxicity of interleukin 2-Pseudomonas exotoxin fusion proteins. |
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| Keywords: | Pseudomonas exotoxin Nucleotide sequence Mutation ADP-ribosylation Cytotoxic activity |
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