| 海带岩藻多糖的分离纯化及结构特性的初步研究 |
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| 引用本文： | 娄翠,汤顺清. 海带岩藻多糖的分离纯化及结构特性的初步研究[J]. 食品工业科技, 2012, 33(4): 135-137,141 |
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| 作者姓名： | 娄翠 汤顺清 |
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| 作者单位： | 1. 暨南大学化学系,广州广东510632／暨南大学生物医学工程系,广州广东510632
2. 暨南大学生物医学工程系,广州广东,510632 |
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| 基金项目： | 2010国家自然科学基金(31070853/C1002) |
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| 摘 要： | 采用稀酸浸提和乙醇沉淀的方法提取海带岩藻多糖(Laminaria japonica Fucoidan,LJF),经DEAE-52分离纯化后,以LJF-2为研究对象,用SephadexG-200凝胶柱色谱、凝胶渗透色谱法和比旋度法对LJF-2的纯度进行鉴定,结果表明LJF-2为均一组分,其分子量为69518。红外光谱仪分析LJF-2有一般糖类物质的特征吸收峰,并且有S=O的吸收峰;X-射线表明LJF-2几乎不含结晶;波谱扫描实验暗示LJF-2在水溶液中可能不具有三股螺旋状构象,LJF-2的特性粘度值较大,表明此多糖在水溶液中具有较高的刚性。
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| 关 键 词： | 海带岩藻多糖 纯化 分子量 |
Purification,separation and structural properties analyses of laminaria japonica fucoidan |
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| LOU Cui,TANG Shun-qing. Purification,separation and structural properties analyses of laminaria japonica fucoidan[J]. Science and Technology of Food Industry, 2012, 33(4): 135-137,141 |
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| Authors: | LOU Cui TANG Shun-qing |
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| Affiliation: | 1.Department of Chemistry,Jinan University,Guangdong 510632,China;2.Department of Biomedical Engineering,College of Life Science,Jinan University,Guangdong 510632,China) |
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| Abstract: | Laminaria japonica fucoidan was extracted by the diluted hydyochloric acid and sedimentated with ethanol.The polysaccharide was perfected by DEAE-52 chromatography.LJF-2 was the research object.The homogeneity of LJF-2 was proved by chromatography of SephadexG-200,GPC and polarimetry respectively.The results showed that the LJF-2 was a single components.The average molecular weight of LJF-2 was 69518.The IR spectra of LJF-2 proved that it existed a general characteristic absorption peak of polysaccharides and S=O absorption peak.The results of XRD showed that LJF-2 was non-crystal.Spectral scanning experiments showed that three strands helical conformation did’t exist in aqueous solution of LJF-2.The intrinsic viscosity of LJF-2 was large,indicating that the polysaccharide existed a high rigidity in aqueous solution. |
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| Keywords: | laminaria japonica fucoidan purification molecular weight |
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