养殖罗非鱼肉中邻苯二甲酸酯残留分析

以美国国家环境保护局列出的6种有内分泌紊乱作用的优先控制污染物邻苯二甲酸酯(phthalic acid esters,PAEs)为目标物,乙腈为提取溶剂,加标鱼肉样品经过超声萃取、OPT固相萃取富集和气相色谱-氢火焰离子化检测器检测,6种PAEs分离特异性好,检出限0.2～3.8μg/kg之间,回收率33.7%～92.9%,相对标准偏差8.7%～14.6%。广东省主要养殖基地的罗非鱼体内PAEs污染主要有邻苯二甲酸二甲酯(DMP)、邻苯二甲酸二异辛酯(DEHP)、邻苯二甲酸二正丁酯(DnBP)和邻苯二甲酸二乙酯(DEP),其中DnBP含量高达38mg/kg(体重,干重),其余三种PAEs的含量在0.2～12.8mg/kg之间。相关分析表明,鱼肉中PAEs的种类、含量与其所处的养殖水中PAEs情况存在显著相关(P<0.01)。养殖水体中PAEs富集是造成鱼类PAEs污染的主要原因之一,建议加强水源质量监控以减少鱼体PAEs污染。

Analysis of phthalate esters residue in the tissue of cultured fishes

Six U.S.EPA priority pollutants of Phthalic acid esters(PAEs) with potential effect on disordered endocrine were adopted as objective compounds.Acetonitrile was selected as extraction agent.Fish meat samples added with PAEs standards were treated by ultrasound radiation,enriched by solid-phase extraction on OPT column and followed by GC-FID detection.Six PAEs were separated well in GC spectra.The low detection limits of PAEs were 0.2～3.8μg/kg.The recoveries of the 6 PAEs were 33.7%～92.9% and the relative standard deviations were in the range of 8.7%～14.6%.The main PAEs pollutants in the fish sample from typical aquatic breeding areas in Guangdong province were DMP,DEHP,DnBP and DEP.The DnBP content in fish sample was as high as 38mg/kg and the other three PAEs content was in the range of 0.2～12.8mg/kg.Correlation analysis indicated that the PAEs category and content in fish sample was closely related to that in the relative breeding aquatic water(P<0.01).The accumulation of PAEs in aquaculture water was one of the crucial reasons for PAEs pollution in fishes.It suggested that measures for monitoring and controlling of water resource should be adopted in order to eliminate PAEs pollution in fishes.