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| 源于鸡蛋清溶菌酶ACE抑制肽的分离纯化及结构鉴定 | |
| 引用本文： | 饶胜其,刘锋锋,居涛,徐雯琪,杨严俊.源于鸡蛋清溶菌酶ACE抑制肽的分离纯化及结构鉴定[J].食品工业科技,2012,33(5):90-94. |
| 作者姓名： | 饶胜其 刘锋锋 居涛 徐雯琪 杨严俊 |
| 作者单位： | 1. 江南大学食品科学与技术国家重点实验室,江苏无锡214122／江南大学食品学院,江苏无锡214122／扬州大学食品科学与工程学院,江苏扬州225127 2. 江南大学食品学院,江苏无锡,214122 3. 江南大学食品科学与技术国家重点实验室,江苏无锡214122／江南大学食品学院,江苏无锡214122 |
| 基金项目： | 国家863计划项目(2007AA10Z330) |
| 摘 要： | 利用胃肠消化酶系水解疏水性氨基酸含量高的蛋清溶菌酶蛋白,筛选能抗胃肠消化的高活性ACE抑制肽。通过超滤、制备型反相高效液相色谱(RP-HPLC)、分析型RP-HPLC纯化、氨基酸组成分析及基质辅助激光解吸电离飞行时间串联质谱(MALDI-TOF-TOF-MS),从胃肠消化水解液中分离鉴定出两种ACE抑制肽Lys-Val-Phe(KVF)和Trp-Ile-Arg(WIR),化学合成该两种三肽后,ACE抑制活性测定结果显示其IC50值分别为14μmol/L与88.5μmol/L。研究结果表明,蛋清溶菌酶蛋白来源的ACE抑制肽有望用于高血压的预防与治疗。 |
| 关 键 词： | 溶菌酶 ACE抑制肽 分离纯化 质谱 |
Isolation and identification of ACE inhibitory peptides from the gastrointestinal digests of egg white lysozyme |
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| RAO Sheng-qi,LIU Feng-feng,JU Tao,XU Wen-qi,YANG Yan-jun.Isolation and identification of ACE inhibitory peptides from the gastrointestinal digests of egg white lysozyme[J].Science and Technology of Food Industry,2012,33(5):90-94. | |
| Authors: | RAO Sheng-qi LIU Feng-feng JU Tao XU Wen-qi YANG Yan-jun |
| Affiliation: | 1,2,*(1.State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi 214122,China;2.School of Food Science and Technology,Jiangnan University,Wuxi 214122,China;3.School of Food Science and Engineering,Yangzhou University,Yangzhou 225127,China) |
| Abstract: | The objective was to screen potent ACE inhibitory peptides,which were resistant to gastrointestinal digestion,from enzymatic hydrolysate of egg white lysozyme by gastrointestinal enzymes.Two angiotensin I-converting enzyme(ACE) inhibitory peptide KVF and WIR,were purified from the gastrointestinal digests of egg white lysozyme by centrifugal ultrafiltration,preparative reverse-phase high-performance liquid chromatography(RP-HPLC) and analytical RP-HPLC,and identified by matrix assisted laser desorption ionization time of flight tandem mass spectrometry(MALDI-TOF-TOF-MS) based on analysis of amino acids composition.After KVF and WIR was chemically synthesized,ACE inhibitory activities(IC50) of these two tripeptides were determined as 14μmol/L and 88.5μmol/L,respectively.The results suggested that the ACE inhibitory peptides from egg white lysozyme may have potential for use in the prevention and treatment of hypertension. |
| Keywords: | lysozyme ACE inhibitory peptide isolation mass spectrometry |
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