响应面法优化超声辅助提取油茶壳中单宁的工艺

研究了以蒸馏水为提取剂从油茶壳中提取单宁的工艺。在单因素实验的基础上,运用Box-Behnken中心组合实验和响应面法考察了液料比、提取温度和提取时间3个因素对单宁含量的影响,并优化了提取工艺。结果表明,最佳的工艺条件为:液料比30(mL.g-1),提取温度63℃,提取时间26min。在此条件下,单宁含量的预测值为4.28%,验证实验值为4.25%,两者相近,说明响应面法优化油茶壳中单宁的提取工艺可行。

Optimization of ultrasonic assisted tannins extraction technology from Camellia oleifera Abel nutshell by response surface methodology

Tannins extracted from Camellia oleifera Abel nutshell were studied using water as the extraction solvent.Based on the single factor test analysis,the effects of three variables(namely liquid-solid ratio,extraction temperature and extraction time)on the tannins yield and the optimum process were evaluated by Box-Behnken central composite experimental design and response surface methodology analysis.The results indicated that liquid-solid ratio of 30(mL·g-1),extraction temperature of 63℃ and extraction time of 26min were the optimum condition.Under these optimized conditions,the experimental values of tannins yield was 4.28%,in good agreement with the predictive value 4.25%.Therefore,the tannins extraction from Camellia oleifera Abel nutshell optimized by response surface methodology was very reliable.