表面印迹法制备链霉素分子印迹聚合物及其性能研究

利用硅胶颗粒为基质,在其表面接枝硅烷化试剂3-甲基丙烯酰氧基丙基三甲氧基硅烷(γ-MPS),进行硅烷化处理后,以链霉素为模板分子,甲基丙烯酸(MAA)为功能单体,N,N’-亚甲基双丙烯酰胺(MBA)为交联剂在颗粒表面合成分子印迹层,制备得到链霉素分子印迹聚合物(MIPMs)和空白聚合物(NMIPMs),并采用静态平衡结合法借助高效液相色谱-蒸发光散射(HPLC-ELSD)研究了聚合物对模板分子链霉素的吸附能力、结合动力学和选择特性。扫描电镜观察和红外光谱分析结果表明表面印迹层已经成功合成;吸附实验结果表明,MIPMs比NMIPMs对链霉素具有更强的吸附特性和更好的选择性。

Study on synthesis and characterization of molecularly imprinted polymers of streptomycin by surface imprinting polymerization

Silicon dioxide(SiO2)particles were treated with silane and their surface were grafted with 3-(methacryloxypropyl)-trimethoxysilane(γ-MPS).Subsequently molecularly imprinted membrane was synthesized on the surface of SiO2 particles,where streptomycin was used as the template molecule,methacrylic acid(MAA)was used as functional monomer and N,N’-Methylenebisacrylamide(MBA)was used as cross linker.Finally,molecularly imprinted polymer(MIPMs)and non-molecularly imprinted polymer(NMIPMs)were prepared by surface imprinting polymerization.The morphology of MIPMs was characterized by means of scanning electron microscopy(SEM).Infrared spectra of MIPMs and NMIPMs demonstrated the components of them.The experimental results of isotherm binding of MIPMs and NMIPMs at different concentrations of streptomycin indicated that MIPMs absorbed more substrates than NMIPMs.MIPMs also showed good selectivity for streptomycin through the isotherm binding test of two other different substrates which were neomycin and gentamicin.