淫羊藿苷预适应对乳鼠心肌细胞的保护作用

目的:探讨淫羊藿苷预适应对培养乳鼠心肌细胞缺氧/复氧损伤和氧化损伤的作用及其机制。方法:体外培养原代乳鼠心肌细胞,淫羊藿苷预适应24h后,采用连二亚硫酸钠(Na2S2O4)诱导心肌细胞缺氧/复氧损伤和过氧化氢(H2O2)诱导氧化损伤模型,测定细胞存活率(MTT法)、细胞培养液中乳酸脱氢酶(LDH)释放量、细胞内超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。结果:在缺氧/复氧损伤模型和氧化损伤模型中,淫羊藿苷预适应明显提高细胞存活率,增加胞内SOD活性,降低培养液中LDH释放量和胞内MDA含量。结论:淫羊藿苷预适应对缺氧/复氧损伤以及氧化损伤心肌细胞都具有明显的保护作用,可能与其抑制脂质过氧化作用有关。

Protective effects of preconditioning of icariin on neonatal rat cardiomyocytes

Aim:To observe the effects of preconditioning of icariin on neonatal rat cardiomyocytes damaged by hypoxia/reoxygenation or by oxidative stress,and study its action mechanisms.Methods:The neonatal rat cardiomyocytes were isolated and cultured in vitro.After 24 hours' preconditioning of icariin,the model of hypoxia-reoxygenation injury was induced by sodium dithionite(Na_2S_2O_4)and the model of oxidative injury was induced by hydrogen peroxide(H_2O_2).In both models,the viability of cardiomyocytes(using MTT method),dehydrogenase(LDH)activities in the medium,superoxide dismutase(SOD)activities and intracellular malonaldehyde(MDA)levels were measured.Results:In the model of hypoxia-reoxygenation injury induced by Na_2S_2O_4 and the model of oxidative injury induced by H_2O_2,preconditioning of icariin could significantly raise the viability of cardiomyocytes and SOD activities,and decreased LDH leakage and intracellular MDA production.Conclusion:Preconditioning of icariin could obviously protect cultured neonatal cardiomyocytes from hypoxia-reoxygenation damages and oxidative damages,and the beneficial effects may be related to its antioxidant properties.