甘蓝型油菜SSR核心引物研究

为了确定一组适用于品种纯度鉴定和遗传多样性分析的SSR核心引物,以100份具有代表性的甘蓝型油菜品种（系）为研究材料对746对SSR引物进行筛选,综合考虑PIC（平均多态信息量）值大小、引物重复性、扩增带型清晰度、连锁群分布等因素,确定44对引物为甘蓝型油菜核心引物,其中20对引物为首选核心引物,24对引物为备选核心引物。20对首选核心引物每对可检测到3~9个多态性位点,PIC值介于0.56~0.80,共检测到102个位点,分布于11个连锁群上。随机抽取1对核心引物对一个杂交组合大田制种F1纯度进行鉴定,其鉴定结果与田间自然鉴定结果一致,并可同时鉴别出来源于父本及外来的混杂单株。将44对核心引物应用于品种的系谱分析和100份品种遗传多样性聚类分析,同样得到了很好的验证结果。该套SSR核心引物适用于甘蓝型油菜品种鉴定及遗传多样性研究。

Screening of Brassica napus core SSR primers

In order to find a set of SSR core primers for purity identification and genetic diversity research on rapeseed,746 SSR primers were screened using 100 representative materials of Brassica napus L.collected from different areas of China.44 primers were selected according to PIC（polymorphism information content） value,reproducibility,patterns and distribution on genome.Among them,20 were determined as preferred core primers,others were candidate core primers.There were 3 to 9 alleles amplified by each pair of preferred primers with PIC values range from 0.56 to 0.80.A total of 102 polymorphism alleles were detected by the preferred SSR core primers.They covered 11 linkage groups of B.napus.The effectiveness of core primers on purity identification and cultivar protection was tested on a randomly selected hybrid F1.Consistent result was obtained between amplification and field identification even by a randomly chosen core primer.And the primer was able to identify the seed from non-female parent.Results from genetic clustering and family origin assay also showed the validity of the core primers for germplasm diversity analysis.