系统性红斑狼疮患者CpG基序甲基化状态与淋巴细胞功能相关抗原-1表达的研究

目的 研究系统性红斑狼疮患者(SLE)CpG基序甲基化状态和淋巴细胞功能相关抗原-1(LFA-1)mRNA的表达水平,并探讨两者的关系.方法 提取26例SLE患者与17名健康人外周血淋巴细胞,分别用5-甲基胞嘧啶抗体与流式细胞仪检测CpG基序甲基化状态和反转录-聚合酶链反应(RT-PCR)分析LFA-1 mRNA表达水平.结果 SLE患者的CpG基序甲基化水平(10.0±1.2)低于健康对照组(11.9±1.0,P<0.05),并与SLE疾病活动指数(SLEDAI)旱负相关(r=-0.62,P<0.05);SLE患者IJFA-1 mRNA表达(0.55±0.11)明显高于健康对照组(0.25±0.08,P<0.05),并与SLEDAI呈正相关(r=0.54,P<0.05);SLE患者CpG基序甲基化水平与LFA-1 mRNA表达呈负相关(r=-0.57,P<0.05).结论 SLE患者存在CpG基序低甲基化状态,并与LFA-1高表达相关联,表观遗传学在SLE发病机制中具有重要作用.

The study of DNA CpG methylation and lymphocyte function-associated antigen-1 expression in patients with systemic lupus erythematosus

Objective To study the methylation of CpG motifs and expression of lymphocyte function-associated antigen-1 (LFA-1) mRNA in patients with systemic lupus erythematosus (SLE), and evaluate their associations. Methods The peripheral blood lymphocytes were collected from 26 patients with SLE and 17 normal individuals. The methylation of CpG motifs was detected by the 5-methylcytosine antibody and flow eytometry, and the expression of LFA-1 mRNA was analyzed by RT-PCR. Results Methylation of CpG motifs in patients with SLE was lower than the control subjects (P<0.05), and a negative correlation existed between methylation of CpG motifs and SLEDAI (P<0.05). While expression of LFA-1 mRNA in patients with SLE was higher than that in the control group (P<0.05), and a positive correlation could be detected between the expression of LFA-1 mRNA and SLEDAI (P<0.05). There was a negative correlation between methylation of CpG motifs and expression of LFA-1 mRNA (P<0.05) in patients with SLE. Conclusion Hypomethylation of CpG motifs does exist in patients with SI,E, and is correlated with high expression of LFA-1, therefore, epige-netics plays an important role in the pathogenesis of SLE.