| 舌鳞癌SENP5的表达及其临床意义 |
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| 引用本文： | 丁小军,孙坚,王丽珍,武和明,李国林,沈毅,李军,周晓健,陈万涛. 舌鳞癌SENP5的表达及其临床意义[J]. 中国口腔颌面外科杂志, 2009, 7(4): 342-346 |
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| 作者姓名： | 丁小军 孙坚 王丽珍 武和明 李国林 沈毅 李军 周晓健 陈万涛 |
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| 作者单位： | 丁小军(上海交通大学医学院附属第九人民医院·口腔医学院口腔颌面外科,上海,200011;上海市口腔医学研究所·上海市口腔医学重点实验室,上海,200011;复旦大学附属中山医院口腔科,上海,200032);孙坚,王丽珍,武和明,李国林,沈毅,李军,周晓健,陈万涛(上海交通大学医学院附属第九人民医院·口腔医学院口腔颌面外科,上海,200011;上海市口腔医学研究所·上海市口腔医学重点实验室,上海,200011) |
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| 基金项目： | 上海市重点(优势)学科建设项目(Y0203)Supported by Shanghai Leading Academic Discipline Project |
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| 摘 要： | 目的：探讨小泛素样修饰物特异的蛋白酶5（SUMO specific protease5，SENP5）在舌鳞状细胞癌中的表达及意义。方法：采用免疫组化方法对33例原发舌鳞癌及癌旁上皮中SENP5的表达进行检测。应用SPSS11．0软件包对免疫组化半定量检测结果与有关临床特征和病理指标间的关系进行Wilcoxon检验和Spearman等级相关检验。另使用钙离子对人舌癌细胞系Tca8113进行诱导分化，实时定量PCR和细胞免疫化学检测SENP5的表达改变。结果：舌鳞癌中SENP5的表达高于癌旁上皮（P=0．000），表达水平与鳞癌的分化程度相关（P=0．022）；钙离子诱导分化可使人舌癌细胞系Tca8113中SENP5的表达增加。结论：SENP5的表达水平与舌鳞癌的分化相关，提示其与肿瘤的发生、发展具有一定关系．
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| 关 键 词： | SENP5 小泛素样修饰物 舌鳞癌 免疫组织化学 实时定量PCR 细胞免疫化学 |
Overexpression of SENP5 in tongue squamous cell carcinoma and the association with differentiation |
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| DING Xiao-jun,SUN Jian,WANG Li-zhen,WU He-ruing,LI Guo-lin,SHEN Yi,LI Jun,ZHOU Xiao-jian,CHEN Wan-tao. Overexpression of SENP5 in tongue squamous cell carcinoma and the association with differentiation[J]. China Journal of Oral and Maxillofacial Surgery, 2009, 7(4): 342-346 |
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| Authors: | DING Xiao-jun SUN Jian WANG Li-zhen WU He-ruing LI Guo-lin SHEN Yi LI Jun ZHOU Xiao-jian CHEN Wan-tao |
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| Affiliation: | 1.Department of Oral and Maxillofaeial Surgery, Ninth People＇s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine.Shanghai 200011;2.Shonghai Key Laboratory of Stomatology ＆ Shanghai Research Institute of Stomatology. Shanghai 200011;3. Department of Stomatology, Zhongshan Hospital, Fudan University. Shanghai 200032, China) |
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| Abstract: | PURPOSE： To analyze the expression of SENP5 in human tongue squamous cell carcinoma and investigate the relationship between its expression and clinieopathological parameters. METHODS： The expression of SENP5 in 33 patients with tongue squamous cell carcinoma was investigated by immunohistochemistry. Immunoreactivity was evaluated semiquantitatively using German immunoreactive score （IRS）. The relationship between SENP5 expression and patients＇ clinical and pathological characteristics was analyzed using Wilcoxon signed rank test and Spearman＇s rank correlation test and survival curves were calculated using the Kaplan-Meier method and Log rank test with SPSS11.0 software package. The change of SENP5 expression in TcaS113 cells induced by calcium shift was detected using real-time PCR and immunocytochemistry. RESULTS： The expression of SENP5 in tongue squamous cell carcinoma was significantly higher than that in paracarcinomous epithelia （P=0.000）. SENP5 expression was not correlated with any clinical pathological parameters other than differentiation of tongue squamous cell carcinoma （P=0.022）. Calcium induction resulted in increased expression of SENP5. CONCLUSIONS： The results show the correlation between SENP5 expression and the differentiation of tongue squamous cell carcinoma. Supported by Shanghai Leading Academic Discipline Project （Grant No. Y0203）. |
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| Keywords: | SENP5 SENPS Small ubiquitin-like modifiers Tongue squamous cell carcinoma Immunohistochemistry Real-time PCR Immunocytochemistry |
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