| 褪黑素对大鼠急性酒精性肝损伤的作用 |
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| 引用本文： | 洪汝涛,陈世林,阮海玲,梅俏,许建明.褪黑素对大鼠急性酒精性肝损伤的作用[J].中国药理学通报,2011,27(11):1596-1599. |
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| 作者姓名： | 洪汝涛 陈世林 阮海玲 梅俏 许建明 |
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| 作者单位： | 安徽医科大学第一附属医院消化内科,安徽省消化病重点实验室,安徽,合肥,230022 |
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| 基金项目： | 安徽省高等学校省级自然科学研究项目 |
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| 摘 要： | 目的探讨褪黑素对大鼠急性酒精性肝损伤的作用及机制。方法采用乙醇灌胃法建立大鼠急性酒精性肝损伤模型,生化法检测血清ALT、AST活性和肝匀浆SOD活性、MDA含量,HE染色观察肝脏病理形态学改变,免疫组化法检测肝组织中TNF-α和IL-1β表达。结果褪黑素干预组血清ALT、AST活性明显低于模型组(分别为P<0.05,P<0.01);褪黑素干预组肝匀浆MDA水平较模型组明显下降(P<0.05),SOD活性较模型组明显升高(P<0.05);光镜下显示褪黑素干预组肝脏病理损伤有所减轻;褪黑素干预组肝组织中TNF-α和IL-1β表达较模型组明显减弱(分别为P<0.05,P<0.01)。结论褪黑素对大鼠酒精所致急性肝损伤具有改善作用,其作用机制可能与抗氧化,以及减少TNF-α和IL-1β的生成有关。
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| 关 键 词： | 褪黑素 酒精性 肝损伤 氧化应激 肿瘤坏死因子-α 白细胞介素-1β |
Effects of melatonin on acute liver injury induced by ethanol in rats |
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| HONG Ru-tao,CHEN Shi-lin,RUAN Hai-ling,MEI Qiao,XU Jian-ming.Effects of melatonin on acute liver injury induced by ethanol in rats[J].Chinese Pharmacological Bulletin,2011,27(11):1596-1599. |
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| Authors: | HONG Ru-tao CHEN Shi-lin RUAN Hai-ling MEI Qiao XU Jian-ming |
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| Affiliation: | HONG Ru-tao,CHEN Shi-lin,RUAN Hai-ling,MEI Qiao,XU Jian-ming(Dept of Gastroenterology,the First Affiliated Hospital of Anhui Medical University,the Key Laboratory ofDigestive Diseases of Anhui,Hefei 230022,China) |
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| Abstract: | Aim To investigate the effects and the mechanisms of melatonin on rat liver injury induced by acute ethanol administration.Methods 24 male SD rats were randomly divided into 3 groups.Acute alcoholic liver injury was induced by gavage with ethanol in SD rats.Serum activities of alanine aminotransferase(ALT),aspartate aminotransferase(AST) were measured with routine laboratory methods using an autoanalyzer.Malondialdehyde(MDA) and superoxide dismutase(SOD) levels in liver homogenates were assayed by spectroph... |
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| Keywords: | melatonin alcoholic hepatic injury oxidative stress tumor necrosis factor-α interleukin-1β |
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