去甲泽拉木醛的免疫抑制作用

目的 :观察去甲泽拉木醛 (T 96)体外免疫抑制及抑制大鼠移植肾排斥的作用强度。方法 :( 1)体外培养BALB/C小鼠脾细胞 ,加有丝分裂原ConA刺激 ,并分别加不同浓度的T 96,用MTT法测定脾细胞的母细胞化情况。 ( 2 )行原位大鼠肾移植并分组 ,分别给予不同剂量的T 96,观察肾移植大鼠的生存期。结果 :T 960 .5mg·L- 1能显著抑制小鼠脾细胞的母细胞化作用 ,与对照组比较P <0 .0 1。T 9610mg·kg- 1·d- 1,2 0mg·kg- 1·d- 1分别延长肾移植大鼠生存期至 ( 14.8±s 1.0 )d和 ( 18.0± 1.5 )d ,与对照组 ( 6.9± 0 .6)d比较差异有非常显著意义 (P <0 .0 1)。T 9610mg·kg- 1·d- 1与泼尼松 10mg·kg- 1·d- 1联合应用延长肾移植大鼠生存期至 ( 32± 6)d。结论 :T 96有强烈抑制小鼠脾细胞母细胞化的作用 ,且有抑制大鼠移植肾的排斥作用 ,抑制作用随剂量加大而增强 ,与泼尼松联合应用 ,更能延长肾移植大鼠的存活时间。

Effect of demethylzeylasteral on immune inhibition

AIM:To observe the inhibiting effect of demethylzeylasteral (T-96) on lymphocytic blastogenesis in vitro and through the survival days of rats transplanted kidney to further explore the practical effect of T-96 on immunosuppression. METHODS: (1)BALB/C mouse spleen cells were cultured in vitro. Various concentrations of T-96 were added into it under the stimulation of Con A mitogen. The degree of inhibition of T-96 to spleen cell blast transformation was measured with MTT method. (2) Rats were randomly divided into groups after kidney transplantation. Different dosages of T-96 were given to each group respectively. The survival time of these groups rats was recorded. RESULTS: T-96 0.5 mg·L -1 could significantly inhibit mouse spleen cell blast transformation, compared with control group (P< 0.01). T-96 10 mg·kg -1·d -1 and 20 mg·kg -1·d -1 given to transplanted rats might prolong their survival time to (14.8±1.0) d and (18.0±1.5) d, respectively, compared with (6.9±0.6) d of control group. The survival time was significant difference(P< 0.01)。After T-96 10 mg·kg -1·d -1 combined with prednisone 10 mg·kg -1·d -1. The survival time could be markedly prolonged to (32±6)d. CONCLUSION:T-96 can strongly inhibit mouse spleen cell blast transformation and inhibit the rejection of kidney trasplantation in rats. The effect will be stronger when the dosage used increases. T-96 10 mg·kg -1·d -1 combined with prednisone 10 mg·kg -1·d -1, the rat survival time may be further significantly prolonged.